Mouse anti-Human Tenascin C (TNC) Antibody | anti-TNC antibody

Tenascin C (TNC)

Cat. Num. AAA397352

• Gene Names: TNC; GP; JI; TN; HXB; GMEM; TN-C; DFNA56; 150-225
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified
• Synonyms: Tenascin C (TNC); anti-TNC antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG1
• Clone Number: T2H5
• Purity/Purification: Purified
• Form/Format: Phosphate buffer, BSA and 0.09 % sodium azide.
• Concentration: 50 ug/ml (varies by lot)
• Sequence Length: 2201

• Applicable Applications for anti-TNC antibody: Immunohistochemistry (IHC) Formalin/Paraffin, Western Blot (WB)
• Application Notes: Dilution: 1:10-1:50
• Immunogen: Purified Tenascin derived from mammary tumour cells
• Positive Control: Colon carcinoma, abortive tissue, hyperproliferative skin, tonsil
• Incubation Time: 60 min at RT
• Pre-Treatment: Unmasking methods for paraffin sections are not established
• Pre-Treatment: Unmasking methods for paraffin sections are not established
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for anti-TNC antibody

The antibody (T2H5) reacts with at least two forms of Tenascin (apparent MW between 210 and 300 kDa), an. During embryonic development Tenascin appears to play a regulatory role in epithelial differentiation. In many mature tissues Tenascin disappears, but reappears in tissue regeneration and repair, as well as in neoplasia. Tenascin expression has been noted in a variety of epithelial neoplasms, including skin-, prostate-, breast- and colon cancer. Tenascin is a high molecular ECM glycoprotein (calculated MW 240 kDa), which contains hexamere repeat-rich single chains. It contains EGF-, Fibronectin III- and Fibronectin-similar structures. During organogenesis it is exprimed temporarily by many organs. During adulthood expression of the protein it reappears only in regeneration, wound healing and in stroma of various tumours. Probably it has an anti-adhesive function, which plays a role during tumour spreading. Tenascin is found in the central and peripheral nerve system, in smooth muscle, tendon and hyper-proliferative skin. Human Tenascin, extracellular matrix protein.

References

1. Le Poole IC, van den Wijngaard RM, Westerhof W, Das PK. Tenascin is overexpressed in vitiligo lesional skin and inhibits melanocyte adhesion. Br J Dermatol. 1997 Aug;137(2):171-8. 2. Le Poole IC, van den Wijngaard RM, Westerhof W, Das PK. Presence of T cells and macrophages in inflammatory vitiligo skin parallels melanocyte disappearance. Am J Pathol. 1996 Apr;148(4):1219-28. 3. Rulo HF, van Vlijmen-Willems IM, Schalkwijk J, Verstraeten AA, van de Kerkhof PC. Normal human skin demonstrates marked site-variation of tenascin expression, not correlated to epidermal proliferation (Ki-67 binding).J Dermatol Sci. 1993 Feb;5(1):54-7. 4. Verstraeten AA, Mackie EJ, Hageman PC, Hilgers J, Schol DJ, De Jongh GJ, Schalkwijk J. Tenascin expression in basal cell carcinoma. Br J Dermatol. 1992 Dec;127(6):571-4. 5. Müller S., Neureiter D., Stolte M. et al. (1991) Virchows Arch. 438; 435-441.

NCBI and Uniprot Product Information

• NCBI GI #: 153946395
• NCBI GeneID: 3371
• NCBI Accession #: NP_002151.2
• NCBI GenBank Nucleotide #: NM_002160.3
• UniProt Accession #: P24821; Q14583; Q15567; Q5T7S3; C9IYT7; C9J575; C9J6D9; C9J848
• Molecular Weight: 171,360 Da
• NCBI Official Full Name: tenascin
• NCBI Official Synonym Full Names: tenascin C
• NCBI Official Symbol: TNC
• NCBI Official Synonym Symbols: GP; JI; TN; HXB; GMEM; TN-C; DFNA56; 150-225
• NCBI Protein Information: tenascin
• UniProt Protein Name: Tenascin
• Protein Family: Tenascin
• UniProt Gene Name: TNC
• UniProt Synonym Gene Names: HXB; TN; TN-C

NCBI Description

This gene encodes an extracellular matrix protein with a spatially and temporally restricted tissue distribution. This protein is homohexameric with disulfide-linked subunits, and contains multiple EGF-like and fibronectin type-III domains. It is implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity, and neuronal regeneration. [provided by RefSeq, Jul 2011]

Uniprot Description

Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. In tumors, stimulates angiogenesis by elongation, migration and sprouting of endothelial cells (PubMed:19884327).