Mouse anti-Human SHIP1 Antibody | anti-SHIP1 antibody

Anti-SHIP1 Purified

Cat. Num. AAA1801528

• Gene Names: INPP5D; SHIP; SHIP1; SHIP-1; hp51CN; SIP-145; p150Ship
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified by Protein-A Affinity Chromatography
• Synonyms: SHIP1; Anti-SHIP1 Purified; inositol polyphosphate-5-phosphatase D; IPP5PD; IP5PD; INPP5D; anti-SHIP1 antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG2a
• Clone Number: SHIP-02
• Specificity: The antibody SHIP-02 reacts with SHIP-1, a phosphoinositide phosphatase largely confined to hematopoietic cells. Multiple forms of SHIP-1 have been reported with molecular weights of 110, 125, 130, 135 and 145 kDa.
• Purity/Purification: Purified by Protein-A Affinity Chromatography
• Form/Format: Phosphate buffered saline (PBS) solution with 15 mM sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1189

• Applicable Applications for anti-SHIP1 antibody: Flow Cytometry (FC/FACS), Western Blot (WB)
• Immunogen: Peptide coresponding to a sequence within N-terminal domain of Human SHIP-1.
• Preparation and Storage: Store at 2-8 degree C.

Western Blot (WB)

(Western blotting analysis (reducing conditions) of human SHIP-1 in whole cell lysate of THP-1 human acute monocytic leukemia cell line.Lane 1: immunostaining with Isotype mouse IgG1 control (PPV-04; 11-458-C100)Lane 2,3: immunostaining with anti-human SHIP-1 (SHIP-01)Lane 4,5: immunostaining with anti-human SHIP-1 (SHIP-02))

Related Product Information for anti-SHIP1 antibody

SHIP-1 (SH2 domain containing inositol phosphatase-1) is a 5'inositol phosphatase that regulates cell responses in lymphocytes and myeloid cells by hydrolyzing the second messenger PI(3,4,5) trisphosphate. SHIP-1 is recruited upon engagement of both inhibitory and activatory receptors, such as FcgammaRIIB, Fcgamma RIII, FcepsilonRI or cytokine and growth factor receptors, and supresses PI3K-dependent signaling, down-regulates cell migration and invasion of transformed cells and phagocytosis. SHIP-1 also serves as a scaffold for the recruitment of other proteins to the plasma membrane.

NCBI and Uniprot Product Information

• NCBI GI #: 64085167
• NCBI GeneID: 3635
• NCBI Accession #: NP_001017915.1
• NCBI GenBank Nucleotide #: NP_001017915.1
• UniProt Accession #: Q92835
• NCBI Official Full Name: phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 isoform a
• NCBI Official Synonym Full Names: inositol polyphosphate-5-phosphatase D
• NCBI Official Symbol: INPP5D
• NCBI Official Synonym Symbols: SHIP; SHIP1; SHIP-1; hp51CN; SIP-145; p150Ship
• NCBI Protein Information: phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1
• UniProt Protein Name: Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1
• UniProt Gene Name: INPP5D
• UniProt Synonym Gene Names: SHIP; SHIP1; SIP-145; SH2 domain-containing inositol phosphatase 1; SHIP-1; hp51CN
• UniProt Entry Name: SHIP1_HUMAN

NCBI Description

This gene is a member of the inositol polyphosphate-5-phosphatase (INPP5) family and encodes a protein with an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal protein interaction domains. Expression of this protein is restricted to hematopoietic cells where its movement from the cytosol to the plasma membrane is mediated by tyrosine phosphorylation. At the plasma membrane, the protein hydrolyzes the 5' phosphate from phosphatidylinositol (3,4,5)-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, thereby affecting multiple signaling pathways. The protein is also partly localized to the nucleus, where it may be involved in nuclear inositol phosphate signaling processes. Overall, the protein functions as a negative regulator of myeloid cell proliferation and survival. Mutations in this gene are associated with defects and cancers of the immune system. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

SHIP: an SH2-containing inositol phosphatase. A hemopoietic-specific phosphatase that regulates cell survival, growth, cell cycle arrest and apoptosis. Hydrolyzes Ins(1,3,4,5)P4 and PtdIns(3,4,5)P3. A cytosolic protein with a SH2 domain in its N-terminus and two NPXY Shc binding motifs at its C-terminus. Upon receptor cross-linking, SHIP is first recruited to the plasma membrane by its SH2, followed by tyrosine phosphorylation on the NPXY motif. Both membrane relocalization and phosphorylation are essential for its regulatory function. Associates with SHC after cytokine stimulation.

Protein type: Motility/polarity/chemotaxis; Phosphatase, lipid; EC 3.1.3.86

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: membrane; cytosol

Molecular Function: protein binding; inositol-polyphosphate 5-phosphatase activity; SH3 domain binding

Biological Process: inositol phosphate metabolic process; apoptosis; phospholipid metabolic process; phosphatidylinositol biosynthetic process; phosphoinositide dephosphorylation; signal transduction; blood coagulation; leukocyte migration; phosphate metabolic process; T cell receptor signaling pathway