Mouse anti-Human Ki-67 Antibody | anti-Ki-67 antibody

Ki-67, Cell Cycle Marker (MIB-1)

Cat. Num. AAA397261

• Gene Names: Mki67; Ki67; Ki-67; D630048A14Rik
• Reactivity: Human
• Applications: Immunohistochemistry
• Synonyms: Ki-67; Cell Cycle Marker (MIB-1); anti-Ki-67 antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG1
• Clone Number: MIB-1
• Form/Format: Solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide.
• Sequence Length: 2938

• Applicable Applications for anti-Ki-67 antibody: Immunohistochemistry (IHC) Formalin/Paraffin
• Application Notes: Working Concentration: (RTU) neat
• Immunogen: Human nuclear Ki-67 antigen
• Positive Control: Breast or colon carcinoma
• Incubation Time: 60 min at RT
• Pre-Treatment: Unmasking fluid G () or C at 90-100 degree C
• Pre-Treatment: Unmasking fluid G () or C at 90-100 degree C
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for anti-Ki-67 antibody

In retrospective studies the intensity of staining and the frequency of Ki-67 expression in cell nuclei was interpreted as a prognostic factor for the malignancy of tumours (Gerdes et al., 1987). Ki-67 Protein is a nuclear protein, which is ubiquitous present during cell division, that means in all phases of mitosis (S1, G1, G2 and M phase). The protein is strongly exprimed in proliferating tissues e.g. embryonal tissue and rapidly growing tumours. Ki-67 nuclear protein.

References

1. Gerdes J., Schwab U., Lemke H., and Stein H. (1983) Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int. J. Cancer 31; 13-20. 2. Gerdes J., Stein H., Pileri S., Rivano M.T., Gobbi M., Ralfkiaer E., Nielsen K.M., Pallesen G., Bartels H., Palestro G., and Delsol G. (1987) Prognostic relevance of the tumour-cell growth fraction in malignant non-Hodgkin's lymphomas. Letter to the editor: Lancet ii, 448-449. 3. Gerdes J., Becker M.H.G., Key G., and Cattoretti G. (1992) Immunohistochemical detection of tumour growth fraction (Ki-67 antigen) in formalin-fixed and routinely processed tissues. J. Pathol., 168; 85-86. 4. Kubbutat M.H.G., Cattoretti G., Gerdes J., Key G., (1994) Comparison of monoclonal antibodies PC10 and MIB-1 on microwave-processed paraffin sections. Cell Proliferation 27; 553-559.

NCBI and Uniprot Product Information

• NCBI GI #: 1177528
• NCBI GeneID: 17345
• UniProt Accession #: Q61769; Q7TSF6; E9PVX6
• Molecular Weight: 324,718 Da
• NCBI Official Full Name: Ki-67
• NCBI Official Synonym Full Names: antigen identified by monoclonal antibody Ki 67
• NCBI Official Symbol: Mki67
• NCBI Official Synonym Symbols: Ki67; Ki-67; D630048A14Rik
• NCBI Protein Information: proliferation marker protein Ki-67; antigen KI-67
• UniProt Protein Name: Proliferation marker protein Ki-67
• UniProt Gene Name: Mki67
• UniProt Synonym Gene Names: Antigen KI-67 homologCurated; Antigen Ki67 homologCurated

Uniprot Description

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (). Does not contribute to the internal structure of mitotic chromosomes (PubMed:26949251). May play a role in chromatin organization (PubMed:26949251). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed.