Mouse anti-Human HEA125 Antibody | anti-HEA125 antibody
HEA125, Epithelium-specific Glycoprotein, Egp34, EpCAM (CD326)
Gene Names
EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
Reactivity
Human
Applications
Immunohistochemistry
Synonyms
HEA125; Epithelium-specific Glycoprotein; Egp34; EpCAM (CD326); anti-HEA125 antibody
Host
Mouse
Reactivity
Human
Isotype
IgG1
Clone Number
HEA125
Form/Format
Solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide and BSA.
Sequence Length
314
Applicable Applications for anti-HEA125 antibody
Immunohistochemistry (IHC) Formalin/Paraffin
Application Notes
Working Concentration: (RTU) neat
Immunogen
Living human colon carcinoma cell line HT-29
Positive Control
Appendix
Incubation Time
60 min at RT
Pre-Treatment
Pre-treatment with pronase 0.1% or unmasking fluid C
Pre-Treatment
Pre-treatment with pronase 0.1% or unmasking fluid C
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for anti-HEA125 antibody
The antibody HEA 125 reacts intensely with all carcinoma derived from the intestinal tract, stomach, pancreas, liver, lung, breast, ovary, thyroid gland, kidney, bladder, prostate and metastases thereof. Stratified squamous epithelia usually stain less intensely then adenocarcinoma. The antibody is suitable for differentiation between carcinoma an non-carcinoma. Keratinizing areas of a tumour mass usually remain unstained as sarcoma, lymphoma, melanoma and neurogenic tumours do. Epithelial cells express a number of characteristic proteins and glycoproteins like cytokeratins, epithelial membrane antigen and epithelium-specific glycoprotein Egp34 (HEA125). The monoclonal antibody HEA125 which has been derived from immunisation of mice with a colon carcinoma cell line shows a broad reactivity pattern. It is labelling most human epithelial cells and epithelium derived tumours. It doesn't react with epidermal keratinocytes, parietal cells, hepatocytes, thymus cortical epithelium, myoepithelia, and non-epithelial tissues. 34 kDa Epithelium-specific membrane glycoprotein HEA 125.
References
1. Moldenhauer G., Momburg F., Möller P., Schwartz, and Hämmerling G.J. (1987) Epithelium-specific surface glycoprotein of Mr 34,000 is a widely distributed human carcinoma marker. Br. J. Cancer 56; 714-721. 2. Momburg F., Moldenhauer G., Hämmerling G.J., and Möller F. (1987) Immunohistochemical study of the expression of a Mr 34,000 human epithelium-specific surface glycoprotein in normal and malignant tissues. Cancer Research 47; 2883-2891. 3. Simon B., Podolsky, D.K., Moldenhauer G., Isselbacher K.J., Gattoni-Celli, S., and Brand S.J. (1990) Epithelial glykoprotein is a member of a family of epithelial cell surface antigens homologous to nidogen, a matrix adhesion protein. Proc. Natl., Acad. Sci. USA 87; 2755 ff. 4. Kemmner W., Moldenhauer G., Schlag P., and Brossmer R. (1992) Separation of tumor cells from a suspension of dissociated human colorectal carcionoma tissue by means of monoclonal antibody-coated magnetic beads. J. Immunol. Methods 147; 197-200. 5. Winter, M. J. et al. (2003) The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology. Am. J. Pathol. 163: 2139-2148.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,932 Da
NCBI Official Full Name
epithelial cell adhesion molecule
NCBI Official Synonym Full Names
epithelial cell adhesion molecule
NCBI Official Symbol
EPCAM
NCBI Official Synonym Symbols
ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
NCBI Protein Information
epithelial cell adhesion molecule
UniProt Protein Name
Epithelial cell adhesion molecule
UniProt Gene Name
EPCAM
UniProt Synonym Gene Names
GA733-2; M1S2; M4S1; MIC18; TACSTD1; TROP1; Ep-CAM; EGP; EGP314; hEGP314
NCBI Description
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
Uniprot Description
May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
Research Articles on HEA125
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Product Notes
The HEA125 epcam (Catalog #AAA397233) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HEA125, Epithelium-specific Glycoprotein, Egp34, EpCAM (CD326) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HEA125 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Formalin/Paraffin. Working Concentration: (RTU) neat. Researchers should empirically determine the suitability of the HEA125 epcam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HEA125, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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