Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Heat shock factor protein 2 (HSF2) Standard Curve.)
Human Heat Shock Factor Protein 2 (HSF2) ELISA Kit | HSF2 elisa kit
Human Heat Shock Factor Protein 2 (HSF2) ELISA Kit
Gene Names
HSF2; HSF 2; HSTF 2
Reactivity
Human
Synonyms
Heat Shock Factor Protein 2 (HSF2); Human Heat Shock Factor Protein 2 (HSF2) ELISA Kit; HSF2; MGC117376; MGC156196; MGC75048; heat shock factor 2; HSF2 elisa kit
Reactivity
Human
Specificity
Human Heat shock factor protein 2 (HSF2) ELISA Kit has high sensitivity and excellent specificity for detection of Human HSF2. No significant cross-reactivity or interference between Human HSF2 and analogues was observed.
Sequence Length
518
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Heat shock factor protein 2 (HSF2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Heat shock factor protein 2 (HSF2) Standard Curve.)
Related Product Information for HSF2 elisa kit
Description: This Human Heat shock factor protein 2 (HSF2) ELISA Kit employs a two-site sandwich ELISA to quantitate HSF2 in samples. An antibody specific for HSF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HSF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSF2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: HSF2, as well as the related gene HSF1, encodes a protein that binds specifically to the heat-shock element and has homology to HSFs of other species. Heat shock transcription factors activate heat-shock response genes under conditions of heat or other stresses. Although the names HSF1 and HSF2 were chosen for historical reasons, these peptides should be referred to as heat-shock transcription factors.Kallio et al. (2002) found that mouse Hsf2 was expressed in all 3 embryonic layers at embryonic day 7.5 and that the head fold was strongly stained at embryonic day 8.5. At later developmental stages, Hsf2 expression was progressively limited to the central nervous system. In adults, Hsf2 expression was detected in spermatocytes and spermatogonia, but not in elongated spermatids, spermatozoa, or Sertoli cells.
Background: HSF2, as well as the related gene HSF1, encodes a protein that binds specifically to the heat-shock element and has homology to HSFs of other species. Heat shock transcription factors activate heat-shock response genes under conditions of heat or other stresses. Although the names HSF1 and HSF2 were chosen for historical reasons, these peptides should be referred to as heat-shock transcription factors.Kallio et al. (2002) found that mouse Hsf2 was expressed in all 3 embryonic layers at embryonic day 7.5 and that the head fold was strongly stained at embryonic day 8.5. At later developmental stages, Hsf2 expression was progressively limited to the central nervous system. In adults, Hsf2 expression was detected in spermatocytes and spermatogonia, but not in elongated spermatids, spermatozoa, or Sertoli cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
heat shock factor protein 2 isoform b
NCBI Official Synonym Full Names
heat shock transcription factor 2
NCBI Official Symbol
HSF2
NCBI Official Synonym Symbols
HSF 2; HSTF 2
NCBI Protein Information
heat shock factor protein 2
UniProt Protein Name
Heat shock factor protein 2
Protein Family
UniProt Gene Name
HSF2
UniProt Synonym Gene Names
HSTF2; HSF 2; HSTF 2
UniProt Entry Name
HSF2_HUMAN
NCBI Description
The protein encoded by this gene belongs to the HSF family of transcription factors that bind specifically to the heat-shock promoter element and activate transcription. Heat shock transcription factors activate heat-shock response genes under conditions of heat or other stresses. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]
Uniprot Description
HSF2: DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. Belongs to the HSF family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 6q22.31
Cellular Component: cytoplasm; nucleus
Molecular Function: protein homodimerization activity; transcription coactivator activity; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis
Research Articles on HSF2
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Product Notes
The Human HSF2 hsf2 (Catalog #AAA9715908) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715908 ELISA Kit recognizes Human HSF2. It is sometimes possible for the material contained within the vial of "Heat Shock Factor Protein 2 (HSF2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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