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Flow Cytometry (FC/FACS) (Flow cytometry analysis (surface staining) of human peripheral blood with anti-CD97 (MEM-180) FITC.)

Mouse anti-Human CD97 Antibody | anti-CD97 antibody

Anti-Hu CD97 FITC

Gene Names
ADGRE5; CD97; TM7LN1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Synonyms
CD97; Anti-Hu CD97 FITC; adhesion G protein-coupled receptor E5; TM7LN1; ADGRE5; anti-CD97 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Isotype
IgG1
Clone Number
MEM-180
Specificity
The antibody MEM-180 recognizes a unique epitope on CD97, a 75-85 kDa surface glycoprotein of G-protein-coupled receptor family, expressed on activated B and T lymphocytes, monocytes/macrophages, dendritic cells and granulocytes.
Purity/Purification
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Form/Format
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Sequence Length
786
Applicable Applications for anti-CD97 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC/FACS: QC tested
Immunogen
PHA-activated peripheral blood cells
Conjugation
FITC
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis (surface staining) of human peripheral blood with anti-CD97 (MEM-180) FITC.)

Flow Cytometry (FC/FACS) (Flow cytometry analysis (surface staining) of human peripheral blood with anti-CD97 (MEM-180) FITC.)
Related Product Information for anti-CD97 antibody
CD97 is a G-protein-coupled seven-span transmembrane adhesive receptor that is constitutively expressed on granulocytes and monocytes and rapidly upregulated on T and B cells upon activation. CD97 is produced in alternatively spliced forms and its cellular ligand is CD55 (DAF), which protects various cell types from complement-mediated damage. Interaction of CD97 on leukocytes and CD55 on vessel cells probably facilitate leukocyte activation and migration into the tissues, similarly, CD97 seems to play a role in tumour migration and invasiveness. CD97 is involved in T cell regulation and peripheral granulocyte homeostasis.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
976
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
adhesion G protein-coupled receptor E5 isoform 3 preproprotein
NCBI Official Synonym Full Names
adhesion G protein-coupled receptor E5
NCBI Official Symbol
ADGRE5
NCBI Official Synonym Symbols
CD97; TM7LN1
NCBI Protein Information
adhesion G protein-coupled receptor E5
UniProt Protein Name
CD97 antigen
Protein Family
UniProt Gene Name
CD97
UniProt Entry Name
CD97_HUMAN

NCBI Description

This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. [provided by RefSeq, Jun 2011]

Uniprot Description

CD97: Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 2; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.12

Cellular Component: extracellular space; focal adhesion; membrane; integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding; transmembrane receptor activity; calcium ion binding

Biological Process: G-protein coupled receptor protein signaling pathway; cell surface receptor linked signal transduction; cell-cell signaling; immune response; cell motility; inflammatory response; cell adhesion

Research Articles on CD97

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Product Notes

The CD97 cd97 (Catalog #AAA1801086) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD97 FITC reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD97 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: QC tested. Researchers should empirically determine the suitability of the CD97 cd97 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD97, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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