Mouse anti-Human CD68 Antibody | anti-CD68 antibody

Mouse anti CD68 (Macrosialin), conjugated to FITC

Cat. Num. AAA570357

• Gene Names: CD68; GP110; LAMP4; SCARD1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD68; Mouse anti CD68 (Macrosialin); conjugated to FITC; anti-CD68 antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG1
• Clone Number: Ki-M7
• Specificity: The CD68 mAb (clone Ki-M7) reacts with human macrosialin.; ; The sensitivity of CD68 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity). For this purpose, a mAb concentration range is selected to include both the saturation point (i.e. the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i.e. the mAb dilution expected to represent the least amount of mAb needed to detect identical percentage of cells). In practice, 50ul of leukocytes containing 10^7 cells/ml are stained with 20ul mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold. In addition and to control lot-to-lot variation, the given lot is compared and adjusted to fluorescence standards with defined intensity.
• Form/Format: Purified by Chromatography, Storage buffer: PBS pH 7.2, 1% BSA, 0.05% NaN3
• Sequence Length: 354

• Applicable Applications for anti-CD68 antibody: Flow Cytometry (FC/FACS)
• Application Notes: Permeabilization and Staining Procedure; - In combination with our Permeabilization Kit FIX&PERM intracellular CD68 can be easily stained in cell suspensions.; - For each sample to be analyzed add 50 ul of whole blood, bone marrow or mononuclear cell suspension in a 5ml tube; - Add 100 ul of Reagent A (Fixation Medium, stored and used at room temperature); - Incubate for 15 minutes at room temperature; - Add 5 ml phosphate buffered saline and centrifuge cells for 5 minutes at 300 g; - Remove supernatant and add to cell pellet 100ul Reagent B (Permeabilization Medium) and 20 ul of the CD68 monoclonal antibody conjugate; - Vortex at low speed for 1-2 seconds; - Incubate for 15 minutes at room temperature; - Wash cells with phosphate buffered saline as described above; - Remove supernatant and resuspend cells in sheath fluid for immediate analysis or resuspend cells in 0.5 ml 1.0 %; formaldehyde and store them at 2-8 degree C in the dark. Analyze fixed cells within 24 hours.
• CE Mark: CE
• Conjugation: FITC
• Preparation and Storage: Monoclonal antibody reagents contain optimal concentrations of affinity-purified antibody. For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8 degree C (DO NOT FREEZE!) and protected from prolonged exposure to light. Stability of the reagent: Please refer to the expiry date printed onto the vial. The use of the reagent after the expiration date is not recommended.

Related Product Information for anti-CD68 antibody

CD68 (macrosialin) is a type-one membrane protein with significant sequence homology to a family of lysosomal-associated glycoproteins (lamp). CD68 molecules are predominantly located
intracellularily in cytoplasmic granules but can also be detected in smaller amounts at the cell surface. Particular strong intracellular expression is observed for monocytes and macrophages. In
addition, Langerhans cells as well as plasmacytoid dendritic cells express clear-cut levels of CD68. Low-level reactivity is also observed with a subset of B lymphocytes and activated T lymphocytes. Oxidized low-density lipoprotein is a ligand for CD68.

The CD68 mAb permits the identification and enumeration of monocytes, Langerhans cells and plasmacytoid dendritic cells (in combination with CD4/CD56 staining) in normal and malignant human blood and bone marrow samples using flow cytometry. Results must be put within the context of other tests as well as the clinical history of the patient by a certified professional before final interpretation. Analysis performed with this antibody should be paralleled by positive and negative controls.

References

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NCBI and Uniprot Product Information

• NCBI GI #: 298665
• NCBI GeneID: 968
• Molecular Weight: 34,654 Da
• NCBI Official Full Name: CD68
• NCBI Official Synonym Full Names: CD68 molecule
• NCBI Official Symbol: CD68
• NCBI Official Synonym Symbols: GP110; LAMP4; SCARD1
• NCBI Protein Information: macrosialin; CD68 antigen; macrophage antigen CD68; scavenger receptor class D, member 1
• UniProt Protein Name: Macrosialin
• Protein Family: Macrosialin
• UniProt Gene Name: CD68
• UniProt Entry Name: CD68_HUMAN

NCBI Description

This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

CD68: Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. Belongs to the LAMP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: membrane; lysosomal membrane; plasma membrane; integral to membrane; endosome membrane