Mouse anti-Human CD4 - HLADR Antibody | anti-CD4 - HLADR antibody

CD4 FITC - HLADR PE

Cat. Num. AAA396147

• Gene Names: CD4; CD4mut
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A/G Chromatography
• Synonyms: CD4 - HLADR; CD4 FITC - HLADR PE; anti-CD4 - HLADR antibody

• Host: Mouse
• Reactivity: Human
• Purity/Purification: Protein A/G Chromatography
• Form/Format: Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein
• Sequence Length: 458

• Applicable Applications for anti-CD4 - HLADR antibody: Flow Cytometry (FC/FACS)
• Immunogen: CD4=Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human perherial blood T lymphocytes.
• Preparation and Storage: Product should be stored at 4-8 degree C. DO NOT FREEZE

Related Product Information for anti-CD4 - HLADR antibody

CD4 identifies human helper/inducer T cells expressing the 60,000 M.W. surface antigen (HLA class II reactive). CD4 is present in low density on monocytes. HLA-DR identifies human B cell and T cell subsets associated with approximately 10% of peripheral blood lymphocytes 28-34,000 M.W. surface antigen, also low density on monocytes and macrophages.

Product Categories/Family for anti-CD4 - HLADR antibody

Bi-Test Reagents (FITC/RPE); Bi-Test (tm) Reagents

NCBI and Uniprot Product Information

• NCBI GI #: 10835167
• NCBI GeneID: 920
• NCBI Accession #: NP_000607.1
• NCBI GenBank Nucleotide #: NM_000616.4
• UniProt Accession #: P01730; Q4ZGK2; Q5U066; Q9UDE5; B2R737; D3DUS5
• Molecular Weight: 51,111 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD4 isoform 1
• NCBI Official Synonym Full Names: CD4 molecule
• NCBI Official Symbol: CD4
• NCBI Official Synonym Symbols: CD4mut
• NCBI Protein Information: T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); T-cell surface antigen T4/Leu-3
• UniProt Protein Name: T-cell surface glycoprotein CD4
• UniProt Gene Name: CD4
• UniProt Entry Name: CD4_HUMAN

NCBI Description

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

Function: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.

Subunit structure: Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI-CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120. Ref.15 Ref.18 Ref.19 Ref.21 Ref.27

Subcellular location: Cell membrane; Single-pass type I membrane protein. Note: Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. Ref.20

Post-translational modification: Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. Ref.16

Polymorphism: The OKT monoclonal antibodies are widely used for the analysis of human peripheral blood T-lymphocytes. OKT4 reacts with T-helper/inducer lymphocytes. The OKT4 epitope of the CD4 cell-surface protein is polymorphic in white, black, and Japanese populations. The variable phenotypic expression is due a CD4 polymorphism. OKT4 positive individuals carry Arg-265 and OKT4 negative individuals carry Trp-265 [

MIM:613949].

Miscellaneous: Primary receptor for HIV-1.

Sequence similarities: Contains 3 Ig-like C2-type (immunoglobulin-like) domains.Contains 1 Ig-like V-type (immunoglobulin-like) domain.