Mouse Z-DNA binding protein 1 (ZBP1) ELISA Kit | ZBP1 elisa kit

Mouse Z-DNA binding protein 1 (ZBP1) ELISA Kit

Cat. Num. AAA732283

• Gene Names: ZBP1; DAI; DLM1; DLM-1; C20orf183
• Reactivity: Mouse
• Synonyms: Z-DNA binding protein 1 (ZBP1); Mouse Z-DNA binding protein 1 (ZBP1) ELISA Kit; ZBP1 elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 250-5000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This ZBP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ZBP. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ZBP1 elisa kit

Principle of the assay: ZBP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ZBP antibody and an ZBP-HRP conjugate. The assay sample and buffer are incubated together with ZBP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ZBP concentration since ZBP from samples and ZBP-HRP conjugate compete for the anti-ZBP antibody binding site. Since the number of sites is limited, as more sites are occupied by ZBP from the sample, fewer sites are left to bind ZBP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ZBP concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ZBP1 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 238550212
• NCBI GeneID: 81030
• NCBI Accession #: NP_001153891.1
• NCBI GenBank Nucleotide #: NM_001160419.2
• Molecular Weight: 38,220 Da
• NCBI Official Full Name: Z-DNA-binding protein 1 isoform d
• NCBI Official Synonym Full Names: Z-DNA binding protein 1
• NCBI Official Symbol: ZBP1
• NCBI Official Synonym Symbols: DAI; DLM1; DLM-1; C20orf183
• NCBI Protein Information: Z-DNA-binding protein 1; DNA-dependent activator of IRFs; DNA-dependent activator of IFN-regulatory factors; tumor stroma and activated macrophage protein DLM-1; DNA-dependent activator of interferon regulatory factors
• UniProt Protein Name: Z-DNA-binding protein 1
• Protein Family: Z-DNA-binding protein
• UniProt Gene Name: ZBP1
• UniProt Synonym Gene Names: C20orf183; DLM1
• UniProt Entry Name: ZBP1_HUMAN

NCBI Description

This gene encodes a Z-DNA binding protein. The encoded protein plays a role in the innate immune response by binding to foreign DNA and inducing type-I interferon production. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

ZBP1: Participates in the detection by the host's innate immune system of DNA from viral, bacterial or even host origin. Plays a role in host defense against tumors and pathogens. Acts as a cytoplasmic DNA sensor which, when activated, induces the recruitment of TBK1 and IRF3 to its C-terminal region and activates the downstream interferon regulatory factor (IRF) and NF-kappa B transcription factors, leading to type-I interferon production. ZBP1-induced NF-kappaB activation probably involves the recruitment of the RHIM containing kinases RIPK1 and RIPK3. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; DNA-binding

Chromosomal Location of Human Ortholog: 20q13.31

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: double-stranded RNA adenosine deaminase activity; protein binding; DNA binding; left-handed Z-DNA binding; RNA binding

Biological Process: metabolic process; positive regulation of interferon type I production; innate immune response