Mouse X-Ray Repair Cross Complementing 5 (XRCC5) ELISA Kit | XRCC5 elisa kit

Mouse X-Ray Repair Cross Complementing 5 (XRCC5) ELISA Kit

Cat. Num. AAA455366

• Gene Names: Xrcc5; Ku80; Ku86; AI314015
• Reactivity: Mouse
• Synonyms: X-Ray Repair Cross Complementing 5 (XRCC5); Mouse X-Ray Repair Cross Complementing 5 (XRCC5) ELISA Kit; XRCC5 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of XRCC5. No significant cross-reactivity or interference between XRCC5 and analogues was observed.
• Sequence Length: 732

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.072ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level XRCC5 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level XRCC5 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for XRCC5 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of XRCC5 in mouse tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to XRCC5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to XRCC5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain XRCC5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of XRCC5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 160333605
• NCBI GeneID: 22596
• NCBI Accession #: NP_033559.2
• NCBI GenBank Nucleotide #: NM_009533.2
• UniProt Accession #: P27641; Q3TE46; Q3TJT0; Q3TN82; Q80UT1; Q8C4N6; Q8K1K7; Q9R169
• Molecular Weight: 83,057 Da
• NCBI Official Full Name: X-ray repair cross-complementing protein 5
• NCBI Official Synonym Full Names: X-ray repair complementing defective repair in Chinese hamster cells 5
• NCBI Official Symbol: Xrcc5
• NCBI Official Synonym Symbols: Ku80; Ku86; AI314015
• NCBI Protein Information: X-ray repair cross-complementing protein 5
• UniProt Protein Name: X-ray repair cross-complementing protein 5
• UniProt Gene Name: Xrcc5
• UniProt Synonym Gene Names: G22p2; CTC85; CTCBF

Uniprot Description

Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.