Mouse Vacuolar Protein Sorting 13D (VPS13D) ELISA Kit | VPS13D elisa kit

Mouse Vacuolar Protein Sorting 13D (VPS13D) ELISA Kit

Cat. Num. AAA2707124

• Reactivity: Mouse
• Synonyms: Vacuolar Protein Sorting 13D (VPS13D); Mouse Vacuolar Protein Sorting 13D (VPS13D) ELISA Kit; VPS13D elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Vacuolar Protein Sorting 13D (VPS13D).; No significant cross-reactivity or interference between Vacuolar Protein Sorting 13D (VPS13D) and analogues was observed.
• Sequence Length: 4363

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vacuolar Protein Sorting 13D (VPS13D) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vacuolar Protein Sorting 13D (VPS13D) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VPS13D elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vacuolar Protein Sorting 13D (VPS13D). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vacuolar Protein Sorting 13D (VPS13D). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vacuolar Protein Sorting 13D (VPS13D), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vacuolar Protein Sorting 13D (VPS13D) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 54607141
• NCBI GeneID: 55187
• NCBI Accession #: NP_060626.2
• NCBI GenBank Nucleotide #: NM_018156.3
• UniProt Accession #: Q5THJ4; Q58F10; Q6MZK9; Q6ZV12; Q709C4; Q709C5; Q86UB4; Q9NSJ3; Q9UIM0; J3KP14
• Molecular Weight: 489,060 Da
• NCBI Official Full Name: vacuolar protein sorting-associated protein 13D isoform 2
• NCBI Official Synonym Full Names: vacuolar protein sorting 13 homolog D
• NCBI Official Symbol: VPS13D
• NCBI Protein Information: vacuolar protein sorting-associated protein 13D
• UniProt Protein Name: Vacuolar protein sorting-associated protein 13D
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: VPS13D

NCBI Description

This gene encodes a protein belonging to the vacuolar-protein-sorting-13 gene family. In yeast, vacuolar-protein-sorting-13 proteins are involved in trafficking of membrane proteins between the trans-Golgi network and the prevacuolar compartment. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

VPS13D: a protein belonging to the vacuolar-protein-sorting-13 gene family. In yeast, vacuolar-protein-sorting-13 proteins are involved in trafficking of membrane proteins between the trans-Golgi network and the prevacuolar compartment. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode distinct isoforms. [provided by RefSeq, Jul 2008]

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 1p36.22-p36.21

Cellular Component: extracellular exosome