Human Sorting Nexin 9 (SNX9) ELISA Kit | SNX9 elisa kit

Human Sorting Nexin 9 (SNX9) ELISA Kit

Cat. Num. AAA2705657

• Gene Names: SNX9; SDP1; WISP; SH3PX1; SH3PXD3A
• Reactivity: Human
• Synonyms: Sorting Nexin 9 (SNX9); Human Sorting Nexin 9 (SNX9) ELISA Kit; SDP1; SH3PX1; SH3PXD3A; WISP; SH3 and PX domain-containing protein 1; SH3 and PX domain-containing protein 3A; SNX9 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Sorting Nexin 9 (SNX9).; No significant cross-reactivity or interference between Sorting Nexin 9 (SNX9) and analogues was observed.
• Sequence Length: 595

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.052ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sorting Nexin 9 (SNX9) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sorting Nexin 9 (SNX9) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SNX9 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sorting Nexin 9 (SNX9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sorting Nexin 9 (SNX9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sorting Nexin 9 (SNX9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sorting Nexin 9 (SNX9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4689258
• NCBI GeneID: 51429
• UniProt Accession #: Q9Y5X1; Q9BSI7; Q9BVM1; Q9UJH6; Q9UP20
• Molecular Weight: 66,592 Da
• NCBI Official Full Name: sorting nexin 9
• NCBI Official Synonym Full Names: sorting nexin 9
• NCBI Official Symbol: SNX9
• NCBI Official Synonym Symbols: SDP1; WISP; SH3PX1; SH3PXD3A
• NCBI Protein Information: sorting nexin-9
• UniProt Protein Name: Sorting nexin-9
• Protein Family: Sorting nexin
• UniProt Gene Name: SNX9
• UniProt Synonym Gene Names: SH3PX1; SH3PXD3A; Protein SDP1

NCBI Description

This gene encodes a member of the sorting nexin family. Members of this family contain a phosphoinositide binding domain, and are involved in intracellular trafficking. The encoded protein does not contain a coiled coil region, like some family members, but does contain a SRC homology domain near its N-terminus. The encoded protein is reported to have a variety of interaction partners, including of adaptor protein 2 , dynamin, tyrosine kinase non-receptor 2, Wiskott-Aldrich syndrome-like, and ARP3 actin-related protein 3. The encoded protein is implicated in several stages of intracellular trafficking, including endocytosis, macropinocytosis, and F-actin nucleation. [provided by RefSeq, Jul 2013]

Uniprot Description

Involved in endocytosis and intracellular vesicle trafficking, both during interphase and at the end of mitosis. Required for efficient progress through mitosis and cytokinesis. Required for normal formation of the cleavage furrow at the end of mitosis. Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Promotes internalization of TNFR. Promotes degradation of EGFR after EGF signaling. Stimulates the GTPase activity of DNM1. Promotes DNM1 oligomerization. Promotes activation of the Arp2/3 complex by WASL, and thereby plays a role in the reorganization of the F-actin cytoskeleton. Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Has lower affinity for membranes enriched in phosphatidylinositol 3-phosphate.