Mouse V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) ELISA Kit | FOS elisa kit

Mouse V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) ELISA Kit

Cat. Num. AAA2703135

• Gene Names: FOS; p55; AP-1; C-FOS
• Reactivity: Mouse
• Synonyms: V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS); Mouse V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) ELISA Kit; Cfos; C-Fos; G0S7; Proto-oncogene c-Fos; G0/G1 Switch Regulatory Protein 7; Cellular oncogene fos; FOS elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS).; No significant cross-reactivity or interference between V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) and analogues was observed.
• Sequence Length: 344

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.063ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FOS elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13325364
• NCBI GeneID: 2353
• UniProt Accession #: P01100; P18849; A8K4E2; B4DQ65
• Molecular Weight: 36,301 Da
• NCBI Official Full Name: V-fos FBJ murine osteosarcoma viral oncogene homolog
• NCBI Official Synonym Full Names: Fos proto-oncogene, AP-1 transcription factor subunit
• NCBI Official Symbol: FOS
• NCBI Official Synonym Symbols: p55; AP-1; C-FOS
• NCBI Protein Information: proto-oncogene c-Fos
• UniProt Protein Name: Proto-oncogene c-Fos
• Protein Family: Proto-oncogene
• UniProt Gene Name: FOS
• UniProt Synonym Gene Names: G0S7

NCBI Description

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008]

Uniprot Description

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.