Mouse Urotensin 2 (UST2) ELISA Kit | UST2 elisa kit

Mouse Urotensin 2 (UST2) ELISA Kit

Cat. Num. AAA2023597

• Gene Names: UTS2; UII; U-II; UCN2; PRO1068
• Reactivity: Mouse
• Synonyms: Urotensin 2 (UST2); Mouse Urotensin 2 (UST2) ELISA Kit; UII; U-II; UCN2; PRO1068; Urotensin-II; UST2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Urotensin 2 (UST2).; No significant cross-reactivity or interference between Urotensin 2 (UST2) and analogues was observed.
• Sequence Length: 139

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 6.17-500pg/mL
• Sensitivity: < 2.55pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Urotensin 2 (UST2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Urotensin 2 (UST2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for UST2 elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Urotensin 2 (UST2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Urotensin 2 (UST2) and unlabeled Urotensin 2 (UST2) (Standards or samples) with the pre-coated antibody specific to Urotensin 2 (UST2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Urotensin 2 (UST2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Urotensin 2 (UST2) in the sample.

Product Categories/Family for UST2 elisa kit

Endocrinology; Neuroscience; Hormone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 5803209
• NCBI GeneID: 10911
• NCBI Accession #: NP_006777.1
• NCBI GenBank Nucleotide #: NM_006786.3
• UniProt Accession #: O95399; Q5H8X7; Q6UXF6; Q9UKP7
• Molecular Weight: 16,029 Da
• NCBI Official Full Name: urotensin-2 isoform b preproprotein
• NCBI Official Synonym Full Names: urotensin 2
• NCBI Official Symbol: UTS2
• NCBI Official Synonym Symbols: UII; U-II; UCN2; PRO1068
• NCBI Protein Information: urotensin-2
• UniProt Protein Name: Urotensin-2
• UniProt Gene Name: UTS2
• UniProt Synonym Gene Names: U-II; UII
• UniProt Entry Name: UTS2_HUMAN

NCBI Description

This gene encodes a mature peptide that is an active cyclic heptapeptide absolutely conserved from lamprey to human. The active peptide acts as a vasoconstrictor and is expressed only in brain tissue. Despite the gene family name similarity, this gene is not homologous to urocortin, a member of the sauvagine/corticotropin-releasing factor/urotensin I family. Most of the proprotein is cleaved to make the mature peptide. Transcript variants encoding different preproprotein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

UTS2: Highly potent vasoconstrictor. Belongs to the urotensin-2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: extracellular space; extracellular region

Molecular Function: hormone activity; receptor binding

Biological Process: response to drug; positive regulation of synaptic transmission, cholinergic; positive regulation of circadian sleep/wake cycle, REM sleep; negative regulation of heart rate; negative regulation of glomerular filtration; positive regulation of collagen biosynthetic process; positive regulation of heart rate; response to testosterone stimulus; positive regulation of vasodilation; negative regulation of insulin secretion; synaptic transmission; elevation of cytosolic calcium ion concentration; positive regulation of fibroblast proliferation; positive regulation of angiogenesis; muscle contraction; regulation of blood pressure; response to hypoxia; negative regulation of blood pressure; positive regulation of cell differentiation; positive regulation of blood pressure