Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse U6 snRNA-associated Sm-like protein LSm5 (LSM5) Standard Curve.)
Mouse U6 snRNA-Associated Sm-Like Protein LSm5 (LSM5) ELISA Kit | LSM5 elisa kit
Mouse U6 snRNA-Associated Sm-Like Protein LSm5 (LSM5) ELISA Kit
Gene Names
Lsm5; 2010208O10Rik; 2310034K10Rik
Reactivity
Mouse
Synonyms
U6 snRNA-Associated Sm-Like Protein LSm5 (LSM5); Mouse U6 snRNA-Associated Sm-Like Protein LSm5 (LSM5) ELISA Kit; LSM5; FLJ12710; YER146W; LSM5 homolog; U6 small nuclear RNA associated; OTTHUMP00000202663; OTTHUMP00000202664; OTTHUMP00000202667; U6 snRNA-associated Sm-like protein; LSM5 elisa kit
Reactivity
Mouse
Specificity
Mouse U6 snRNA-associated Sm-like protein LSm5 (LSM5) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse LSM5. No significant cross-reactivity or interference between Mouse LSM5 and analogues was observed.
Sequence Length
91
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse U6 snRNA-associated Sm-like protein LSm5 (LSM5) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse U6 snRNA-associated Sm-like protein LSm5 (LSM5) Standard Curve.)
Related Product Information for LSM5 elisa kit
Description: This Mouse U6 snRNA-associated Sm-like protein LSm5 (LSM5) ELISA Kit employs a two-site sandwich ELISA to quantitate LSM5 in samples. An antibody specific for LSM5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LSM5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LSM5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LSM5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family. Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.Disruption of genes encoding Sm-like proteins directly associated with the U6 snRNA (Lsm2-8) generated variable phenotypes. Lsm2, Lsm3, Lsm4, and Lsm8 are essential for vegetative growth. Lsm5, Lsm6, and Lsm7 are not essential for growth; however, their disruptions lead to slow growth especially at elevated temperature. The levels of the U6 snRNA were strongly reduced in the strains harboring the Lsm5, Lsm6, and Lsm7 disruptions.
Background: Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family. Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.Disruption of genes encoding Sm-like proteins directly associated with the U6 snRNA (Lsm2-8) generated variable phenotypes. Lsm2, Lsm3, Lsm4, and Lsm8 are essential for vegetative growth. Lsm5, Lsm6, and Lsm7 are not essential for growth; however, their disruptions lead to slow growth especially at elevated temperature. The levels of the U6 snRNA were strongly reduced in the strains harboring the Lsm5, Lsm6, and Lsm7 disruptions.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
U6 snRNA-associated Sm-like protein LSm5
NCBI Official Synonym Full Names
LSM5 homolog, U6 small nuclear RNA and mRNA degradation associated
NCBI Official Symbol
Lsm5
NCBI Official Synonym Symbols
2010208O10Rik; 2310034K10Rik
NCBI Protein Information
U6 snRNA-associated Sm-like protein LSm5
UniProt Protein Name
U6 snRNA-associated Sm-like protein LSm5
Protein Family
UniProt Gene Name
Lsm5
UniProt Entry Name
LSM5_MOUSE
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Product Notes
The Mouse LSM5 lsm5 (Catalog #AAA9718076) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718076 ELISA Kit recognizes Mouse LSM5. It is sometimes possible for the material contained within the vial of "U6 snRNA-Associated Sm-Like Protein LSm5 (LSM5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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