Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse U3 small nucleolar ribonucleoprotein protein MPP10 (MPHOSPH10) Standard Curve.)
Mouse U3 Small Nucleolar Ribonucleoprotein Protein MPP10 (MPHOSPH10) ELISA Kit | MPHOSPH10 elisa kit
Mouse U3 Small Nucleolar Ribonucleoprotein Protein MPP10 (MPHOSPH10) ELISA Kit
Gene Names
Mphosph10; AI326008; 2810453H10Rik; 5730405D16Rik
Reactivity
Mouse
Synonyms
U3 Small Nucleolar Ribonucleoprotein Protein MPP10 (MPHOSPH10); Mouse U3 Small Nucleolar Ribonucleoprotein Protein MPP10 (MPHOSPH10) ELISA Kit; MPHOSPH10; CT90; MPP10; MPP10P; M-phase phosphoprotein 10; MPHOSPH10 elisa kit
Reactivity
Mouse
Specificity
Mouse U3 small nucleolar ribonucleoprotein protein MPP10 (MPHOSPH10) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MPHOSPH10. No significant cross-reactivity or interference between Mouse MPHOSPH10 and analogues was observed.
Sequence Length
681
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse U3 small nucleolar ribonucleoprotein protein MPP10 (MPHOSPH10) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse U3 small nucleolar ribonucleoprotein protein MPP10 (MPHOSPH10) Standard Curve.)
Related Product Information for MPHOSPH10 elisa kit
Description: This Mouse U3 small nucleolar ribonucleoprotein protein MPP10 (MPHOSPH10) ELISA Kit employs a two-site sandwich ELISA to quantitate MPHOSPH10 in samples. An antibody specific for MPHOSPH10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MPHOSPH10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPHOSPH10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPHOSPH10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MPHOSPH10 encodes a protein that is phosphorylated during mitosis. The M-phase phosphoprotein 10 localizes to the nucleolus during interphase and to the chromosomes during M phase. The protein associates with the U3 small nucleolar ribonucleoprotein 60-80S complexes and may be involved in pre-rRNA processing.
Background: MPHOSPH10 encodes a protein that is phosphorylated during mitosis. The M-phase phosphoprotein 10 localizes to the nucleolus during interphase and to the chromosomes during M phase. The protein associates with the U3 small nucleolar ribonucleoprotein 60-80S complexes and may be involved in pre-rRNA processing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
U3 small nucleolar ribonucleoprotein protein MPP10
NCBI Official Synonym Full Names
M-phase phosphoprotein 10 (U3 small nucleolar ribonucleoprotein)
NCBI Official Symbol
Mphosph10
NCBI Official Synonym Symbols
AI326008; 2810453H10Rik; 5730405D16Rik
NCBI Protein Information
U3 small nucleolar ribonucleoprotein protein MPP10
UniProt Protein Name
U3 small nucleolar ribonucleoprotein protein MPP10
UniProt Gene Name
Mphosph10
UniProt Entry Name
MPP10_MOUSE
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Product Notes
The Mouse MPHOSPH10 mphosph10 (Catalog #AAA9717588) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717588 ELISA Kit recognizes Mouse MPHOSPH10. It is sometimes possible for the material contained within the vial of "U3 Small Nucleolar Ribonucleoprotein Protein MPP10 (MPHOSPH10), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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