Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tumor necrosis factor ligand superfamily member 9 (TNFSF9) Standard Curve.)
Mouse Tumor Necrosis Factor Ligand Superfamily Member 9 (TNFSF9) ELISA Kit | TNFSF9 elisa kit
Mouse Tumor Necrosis Factor Ligand Superfamily Member 9 (TNFSF9) ELISA Kit
Gene Names
Tnfsf9; Ly63l; 4-1BBL; Cd137l; 4-1BB-L; AI848817
Reactivity
Mouse
Synonyms
Tumor Necrosis Factor Ligand Superfamily Member 9 (TNFSF9); Mouse Tumor Necrosis Factor Ligand Superfamily Member 9 (TNFSF9) ELISA Kit; TNFSF9; 4-1BB-L; CD137L; homolog of mouse 4-1BB-L; receptor 4-1BB ligand; TNFSF9 elisa kit
Reactivity
Mouse
Specificity
Mouse Tumor necrosis factor ligand superfamily member 9 (TNFSF9) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TNFSF9. No significant cross-reactivity or interference between Mouse TNFSF9 and analogues was observed.
Sequence Length
309
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tumor necrosis factor ligand superfamily member 9 (TNFSF9) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Tumor necrosis factor ligand superfamily member 9 (TNFSF9) Standard Curve.)
Related Product Information for TNFSF9 elisa kit
Description: This Mouse Tumor necrosis factor ligand superfamily member 9 (TNFSF9) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFSF9 in samples. An antibody specific for TNFSF9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNFSF9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFSF9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFSF9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TNFSF9 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.
Background: TNFSF9 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 9
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 9
NCBI Official Symbol
Tnfsf9
NCBI Official Synonym Symbols
Ly63l; 4-1BBL; Cd137l; 4-1BB-L; AI848817
NCBI Protein Information
tumor necrosis factor ligand superfamily member 9
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 9
Protein Family
UniProt Gene Name
Tnfsf9
UniProt Synonym Gene Names
Cd137l; Cd157l; Ly63l; 4-1BBL
UniProt Entry Name
TNFL9_MOUSE
Uniprot Description
TNFL9: Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages. Belongs to the tumor necrosis factor family.
Protein type: Apoptosis; Cytokine; Membrane protein, integral; Cell cycle regulation
Cellular Component: extracellular space; membrane; integral to membrane; plasma membrane
Molecular Function: cytokine activity; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding; receptor binding
Biological Process: positive regulation of interferon-gamma production; positive regulation of cytotoxic T cell differentiation; cell-cell signaling; positive regulation of interleukin-12 production; positive regulation of interleukin-6 production; myeloid dendritic cell differentiation; immune response; positive regulation of activated T cell proliferation; regulation of cell proliferation
Research Articles on TNFSF9
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Product Notes
The Mouse TNFSF9 tnfsf9 (Catalog #AAA9716723) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716723 ELISA Kit recognizes Mouse TNFSF9. It is sometimes possible for the material contained within the vial of "Tumor Necrosis Factor Ligand Superfamily Member 9 (TNFSF9), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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