Mouse Tumor Necrosis Factor Alpha (TNFa) ELISA Kit | TNFa elisa kit

Mouse Tumor Necrosis Factor Alpha (TNFa) Mini Samples ELISA Kit

Cat. Num. AAA2708267

• Gene Names: Tnf; DIF; Tnfa; TNF-a; TNFSF2; Tnlg1f; Tnfsf1a; TNFalpha; TNF-alpha
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Tumor Necrosis Factor Alpha (TNFa); Mouse Tumor Necrosis Factor Alpha (TNFa) Mini Samples ELISA Kit; Tumor Necrosis Factor Alpha; DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2; TNFa elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Tumor Necrosis Factor Alpha (TNFa).; No significant cross-reactivity or interference between Mini Samples Tumor Necrosis Factor Alpha (TNFa) and analogues was observed.
• Sequence Length: 219

• Applicable Applications for TNFa elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 15.6-1,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 6.0pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Tumor Necrosis Factor Alpha (TNFa) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Tumor Necrosis Factor Alpha (TNFa) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Tumor Necrosis Factor Alpha (TNFa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Tumor Necrosis Factor Alpha (TNFa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Tumor Necrosis Factor Alpha (TNFa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mini Samples Tumor Necrosis Factor Alpha (TNFa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 518831589
• NCBI GeneID: 21926
• NCBI Accession #: NP_001265530.1
• NCBI GenBank Nucleotide #: NP_001265530.1
• UniProt Accession #: P06804
• NCBI Official Full Name: tumor necrosis factor isoform 2
• NCBI Official Synonym Full Names: tumor necrosis factor
• NCBI Official Symbol: Tnf
• NCBI Official Synonym Symbols: DIF; Tnfa; TNF-a; TNFSF2; Tnlg1f; Tnfsf1a; TNFalpha; TNF-alpha
• NCBI Protein Information: tumor necrosis factor
• UniProt Protein Name: Tumor necrosis factor
• UniProt Gene Name: Tnf
• UniProt Synonym Gene Names: Tnfa; Tnfsf2; TNF-a; NTF; ICD1; ICD2
• UniProt Entry Name: TNFA_MOUSE

NCBI Description

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. Members of this family are classified based on primary sequence, function, and structure. This protein is synthesized as a type-II transmembrane protein and is reported to be cleaved into products that exert distinct biological functions. It plays an important role in the innate immune response as well as regulating homeostasis but is also implicated in diseases of chronic inflammation. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Uniprot Description

TNF-a: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Homotrimer. Interacts with SPPL2B. Belongs to the tumor necrosis factor family.

Protein type: Apoptosis; Cytokine; Membrane protein, integral; Motility/polarity/chemotaxis

Cellular Component: cell surface; external side of plasma membrane; extracellular space; integral to membrane; integral to plasma membrane; intracellular; lipid raft; phagocytic cup; plasma membrane; recycling endosome; secretory granule

Molecular Function: cytokine activity; identical protein binding; protease binding; protein binding; tumor necrosis factor receptor binding

Biological Process: activation of MAPK activity; activation of MAPKKK activity; activation of NF-kappaB transcription factor; apoptosis; calcium-mediated signaling; caspase activation; cell activation; cell proliferation; cellular extravasation; chronic inflammatory response to antigenic stimulus; cortical actin cytoskeleton organization and biogenesis; defense response; defense response to bacterium; defense response to Gram-positive bacterium; detection of mechanical stimulus involved in sensory perception of pain; DNA damage response, signal transduction resulting in induction of apoptosis; extracellular matrix organization and biogenesis; glucose metabolic process; humoral immune response; induction of apoptosis via death domain receptors; inflammatory response; JNK cascade; leukocyte migration; leukocyte tethering or rolling; lipopolysaccharide-mediated signaling pathway; MAPKKK cascade; multicellular organismal development; negative regulation of cell proliferation; negative regulation of cytokine secretion during immune response; negative regulation of endothelial cell proliferation; negative regulation of glucose import; negative regulation of interleukin-6 production; negative regulation of L-glutamate transport; negative regulation of lipid catabolic process; negative regulation of mitotic cell cycle; negative regulation of myelination; negative regulation of myoblast differentiation; negative regulation of osteoblast differentiation; negative regulation of protein complex disassembly; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of viral genome replication; organ morphogenesis; osteoclast differentiation; positive regulation of action potential; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of cell adhesion; positive regulation of cell proliferation; positive regulation of chemokine biosynthetic process; positive regulation of chemokine production; positive regulation of chronic inflammatory response to antigenic stimulus; positive regulation of cytokine production; positive regulation of cytokine secretion; positive regulation of fever; positive regulation of hair follicle development; positive regulation of heterotypic cell-cell adhesion; positive regulation of humoral immune response mediated by circulating immunoglobulin; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of inflammatory response; positive regulation of interferon-gamma production; positive regulation of interleukin-18 production; positive regulation of interleukin-6 production; positive regulation of interleukin-8 biosynthetic process; positive regulation of interleukin-8 production; positive regulation of JNK activity; positive regulation of JNK cascade; positive regulation of MAP kinase activity; positive regulation of membrane protein ectodomain proteolysis; positive regulation of mitosis; positive regulation of neuron apoptosis; positive regulation of NF-kappaB import into nucleus; positive regulation of NFAT protein import into nucleus; positive regulation of nitric oxide biosynthetic process; positive regulation of osteoclast differentiation; positive regulation of peptidyl-serine phosphorylation; positive regulation of phagocytosis; positive regulation of programmed cell death; positive regulation of protein amino acid phosphorylation; positive regulation of protein complex assembly; positive regulation of protein complex disassembly; positive regulation of protein kinase activity; positive regulation of protein kinase B signaling cascade; positive regulation of protein transport; positive regulation of smooth muscle cell proliferation; positive regulation of synaptic transmission; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of translational initiation by iron; protein import into nucleus, translocation; protein kinase B signaling cascade; receptor biosynthetic process; regulation of cell proliferation; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of immunoglobulin secretion; regulation of insulin secretion; regulation of osteoclast differentiation; regulation of protein amino acid phosphorylation; regulation of protein secretion; response to glucocorticoid stimulus; response to lipopolysaccharide; response to organic substance; response to virus; sequestering of triacylglycerol; signal transduction; toll-like receptor 3 signaling pathway; tumor necrosis factor-mediated signaling pathway