Western Blot (WB)
(The extracts of mouse muscle (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TNNI1 antibody (1:500~5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: TNNI1 mAb 1:500 Lane 2.: TNNI1 mAb 1:1000 Lane 3.: TNNI1 mAb 1:5000)
Mouse anti-Human TNNI1 Monoclonal Antibody | anti-TNNI1 antibody
TNNI1
Western Blot (WB)
(The extracts of mouse muscle (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TNNI1 antibody (1:500~5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: TNNI1 mAb 1:500 Lane 2.: TNNI1 mAb 1:1000 Lane 3.: TNNI1 mAb 1:5000)
Western Blot (WB)
(The extracts of mouse muscle (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TNNI1 antibody (1:500~5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: TNNI1 mAb 1:500 Lane 2.: TNNI1 mAb 1:1000 Lane 3.: TNNI1 mAb 1:5000)
NCBI and Uniprot Product Information
NCBI Description
Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes. [provided by RefSeq, Jul 2008]
Uniprot Description
TNNI1: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Belongs to the troponin I family.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: 1q31.3
Cellular Component: troponin complex; cytosol
Molecular Function: metal ion binding; actin binding
Biological Process: regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; muscle filament sliding
Research Articles on TNNI1
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Product Notes
The TNNI1 tnni1 (Catalog #AAA201201) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TNNI1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TNNI1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Researchers should empirically determine the suitability of the TNNI1 tnni1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNNI1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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