Mouse Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) ELISA Kit | TREM2 elisa kit

Mouse Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) Wide-range ELISA Kit

Cat. Num. AAA2709326

• Gene Names: Trem2; TREM-2; Trem2a; Trem2b; Trem2c
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Triggering Receptor Expressed On Myeloid Cells 2 (TREM2); Mouse Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) Wide-range ELISA Kit; Triggering Receptor Expressed On Myeloid Cells 2; Trem2a; Trem2b; Trem2c; Triggering receptor expressed on monocytes 2; TREM2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Wide-range Triggering Receptor Expressed On Myeloid Cells 2 (TREM2).; No significant cross-reactivity or interference between Wide-range Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) and analogues was observed.
• Sequence Length: 249

• Applicable Applications for TREM2 elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Detection Range: 78-5,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 32pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wide-range Triggering Receptor Expressed On Myeloid Cells 2 (TREM2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wide-range Triggering Receptor Expressed On Myeloid Cells 2 (TREM2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wide-range Triggering Receptor Expressed On Myeloid Cells 2 (TREM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Wide-range Triggering Receptor Expressed On Myeloid Cells 2 (TREM2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 440918700
• NCBI GeneID: 83433
• NCBI Accession #: NP_001259007.1
• NCBI GenBank Nucleotide #: NP_001259007.1
• UniProt Accession #: Q99NH8
• NCBI Official Full Name: triggering receptor expressed on myeloid cells 2 isoform 2
• NCBI Official Synonym Full Names: triggering receptor expressed on myeloid cells 2
• NCBI Official Symbol: Trem2
• NCBI Official Synonym Symbols: TREM-2; Trem2a; Trem2b; Trem2c
• NCBI Protein Information: triggering receptor expressed on myeloid cells 2
• UniProt Protein Name: Triggering receptor expressed on myeloid cells 2
• Protein Family: Triggering receptor expressed on myeloid cells
• UniProt Gene Name: Trem2
• UniProt Synonym Gene Names: Trem2a; Trem2b; Trem2c; TREM-2
• UniProt Entry Name: TREM2_MOUSE

NCBI Description

The protein encoded by this gene is part of the immunoglobulin and lectin-like superfamily and functions as part of the innate immune system. This gene forms part of a cluster of genes on mouse chromosome 17 thought to be involved in innate immunity. This protein associates with the adaptor protein Dap-12 and recruits several factors, such as kinases and phospholipase C-gamma, to form a receptor signaling complex that activates myeloid cells, including dendritic cells and microglia. In humans homozygous loss-of-function mutations in this gene cause Nasu-Hakola disease and mutations in this gene may be risk factors to the development of Alzheimer's disease. In mouse mutations of this gene serve as a pathophysiological model for polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (Nasu-Hakola disease) and for inflammatory bowel disease. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]

Uniprot Description

TREM2: May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL); also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: membrane; intracellular membrane-bound organelle; extracellular region; integral to membrane; plasma membrane

Molecular Function: peptidoglycan binding; protein binding; transmembrane receptor activity; lipopolysaccharide binding

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; detection of lipopolysaccharide; detection of peptidoglycan; lipopolysaccharide-mediated signaling pathway; innate immune response; positive regulation of calcium-mediated signaling; signal transduction; positive regulation of antigen processing and presentation of peptide antigen via MHC class II