Mouse Transportin 1 (TNPO1) ELISA Kit | TNPO1 elisa kit

Mouse Transportin 1 (TNPO1) ELISA Kit

Cat. Num. AAA456360

• Gene Names: Tnpo1; MIP; TRN; IPO2; MIP1; Kpnb2; AU021749; D13Ertd688e
• Reactivity: Mouse
• Synonyms: Transportin 1 (TNPO1); Mouse Transportin 1 (TNPO1) ELISA Kit; TNPO1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of TNPO1. No significant cross-reactivity or interference between TNPO1 and analogues was observed.
• Sequence Length: 890

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 1.56-100ng/mL
• Sensitivity: 0.39ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TNPO1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TNPO1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Standard Curve/Testing Data

Related Product Information for TNPO1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TNPO1 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNPO1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TNPO1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNPO1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TNPO1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 115385966
• NCBI GeneID: 238799
• NCBI Accession #: NP_001041732.1
• NCBI GenBank Nucleotide #: NM_001048267.1
• UniProt Accession #: Q8BFY9; Q8C0S3; Q8K2E7
• Molecular Weight: 101,312 Da
• NCBI Official Full Name: transportin-1 isoform 2
• NCBI Official Synonym Full Names: transportin 1
• NCBI Official Symbol: Tnpo1
• NCBI Official Synonym Symbols: MIP; TRN; IPO2; MIP1; Kpnb2; AU021749; D13Ertd688e
• NCBI Protein Information: transportin-1
• UniProt Protein Name: Transportin-1
• UniProt Gene Name: Tnpo1
• UniProt Synonym Gene Names: Kpnb2

Uniprot Description

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (). Involved in nuclear import of M9-containing proteins. In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. Appears also to be involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A (). In vitro, mediates nuclear import of SRP19 () and H2A, H2B, H3 and H4 histones. Mediates nuclear import of ADAR/ADAR1 in a RanGTP-dependent manner ().