Mouse Transforming Growth Factor Alpha (TGFa) ELISA Kit | TGFa elisa kit

Mouse Transforming Growth Factor Alpha (TGFa) ELISA Kit

Cat. Num. AAA455396

• Gene Names: Tgfa; wa1; wa-1
• Reactivity: Mouse
• Synonyms: Transforming Growth Factor Alpha (TGFa); Mouse Transforming Growth Factor Alpha (TGFa) ELISA Kit; TGFa elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of TGFA. No significant cross-reactivity or interference between TGFA and analogues was observed.
• Sequence Length: 159

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 15.625-1000pg/mL
• Sensitivity: 5.5pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TGFA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TGFA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TGFa elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TGFA in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGFA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TGFA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TGFA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TGFA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13654266
• NCBI GeneID: 21802
• NCBI Accession #: NP_112476.1
• NCBI GenBank Nucleotide #: NM_031199.4
• UniProt Accession #: P48030
• Molecular Weight: 17,018 Da
• NCBI Official Full Name: protransforming growth factor alpha preproprotein
• NCBI Official Synonym Full Names: transforming growth factor alpha
• NCBI Official Symbol: Tgfa
• NCBI Official Synonym Symbols: wa1; wa-1
• NCBI Protein Information: protransforming growth factor alpha
• UniProt Protein Name: Protransforming growth factor alpha
• Protein Family: Transforming growth factor
• UniProt Gene Name: Tgfa
• UniProt Synonym Gene Names: TGF-alpha; ETGF

NCBI Description

This gene encodes a member of the epidermal growth factor (EGF) family of proteins that regulate cellular proliferation. The encoded protein undergoes proteolytic processing to generate a soluble glycoprotein that is secreted by the cell. The secreted protein binds to the EGF receptors to initiate signaling events resulting in cellular proliferation, mucous production or inhibition of gastric acid secretion. The transgenic expression of the encoded protein in mice induces the development of cancers in various tissues such as liver, pancreas, skin and mammary glands. Mice lacking the encoded protein exhibit a wavy coat and curly whiskers phenotype as well as abnormalities in the eye. [provided by RefSeq, Sep 2015]

Uniprot Description

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.