Mouse TPO ELISA Kit | TPO elisa kit

Mouse TPO (Thrombopoietin) ELISA Kit

Cat. Num. AAA2510249

• Gene Names: TPO; MSA; TPX; TDH2A
• Reactivity: Mouse
• Synonyms: TPO; Mouse TPO (Thrombopoietin) ELISA Kit; TPO elisa kit

• Reactivity: Mouse
• Specificity: This kit recognizes natural and recombinant Mouse TPO. No significant cross-reactivity or interference between Mouse TPO and analogues was observed.
• Sequence Length: 889

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 7.813-500pg/mL
• Sensitivity: Min: 4.688pg/mL; Max: 500pg/mL
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse TPO concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TPO elisa kit

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TPO. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TPO and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TPO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TPO. You can calculate the concentration of TPO in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 28558988
• NCBI GeneID: 7173
• NCBI Accession #: NP_783652.1
• NCBI GenBank Nucleotide #: NM_175721.3
• UniProt Accession #: P07202; P09934; P09935; Q8IUL0; Q8NF94; Q8NF95; Q8NF96; Q8NF97; Q8TCI9
• Molecular Weight: 92,063 Da
• NCBI Official Full Name: thyroid peroxidase isoform d
• NCBI Official Synonym Full Names: thyroid peroxidase
• NCBI Official Symbol: TPO
• NCBI Official Synonym Symbols: MSA; TPX; TDH2A
• NCBI Protein Information: thyroid peroxidase; thyroid microsomal antigen; thyroperoxidase
• UniProt Protein Name: Thyroid peroxidase
• Protein Family: Thyroid peroxidase
• UniProt Gene Name: TPO
• UniProt Synonym Gene Names: TPO
• UniProt Entry Name: PERT_HUMAN

NCBI Description

This gene encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, May 2011]

Uniprot Description

TPO: Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4). An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease. Defects in TPO are the cause of thyroid dyshormonogenesis 2A (TDH2A). A disorder due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete. Belongs to the peroxidase family. XPO subfamily. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Oxidoreductase; Membrane protein, integral; EC 1.11.1.8; Amino Acid Metabolism - tyrosine

Chromosomal Location of Human Ortholog: 2p25

Cellular Component: extracellular space; cell surface; mitochondrion; integral to plasma membrane; plasma membrane

Molecular Function: peroxidase activity; heme binding; iodide peroxidase activity; calcium ion binding

Biological Process: hydrogen peroxide catabolic process; embryonic hemopoiesis; thyroid hormone generation; response to oxidative stress; hormone biosynthetic process

Disease: Thyroid Dyshormonogenesis 2a