Mouse TP53 ELISA Kit | TP53 elisa kit

Mouse TP53 (Tumor Protein 53) ELISA Kit

Cat. Num. AAA2502214

• Reactivity: Mouse
• Synonyms: TP53; Mouse TP53 (Tumor Protein 53) ELISA Kit; TP53 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse TP53. No significant cross-reactivity or interference between Mouse TP53 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse TP53 and all the analogues, therefore, cross reaction may still exist.
• Sequence Length: 45

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 62.5-4000pg/mL
• Sensitivity: Min: 37.5pg/mL; Max: 4000pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TP53 elisa kit

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TP53 in Mouse serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TP53. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse TP53 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TP53, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse TP53. You can calculate the concentration of Mouse TP53 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 557786681
• Molecular Weight: 5,085 Da
• NCBI Official Full Name: TP53, partial
• UniProt Protein Name: TP53
• Protein Family: TP53-regulating kinase
• UniProt Gene Name: TP53
• UniProt Entry Name: U6BHU6_HUMAN

NCBI Description

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013]