Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Thyroid receptor-interacting protein 6 (TRIP6) Standard Curve.)
Mouse Thyroid Receptor-Interacting Protein 6 (TRIP6) ELISA Kit | TRIP6 elisa kit
Mouse Thyroid Receptor-Interacting Protein 6 (TRIP6) ELISA Kit
Reactivity
Mouse
Synonyms
Thyroid Receptor-Interacting Protein 6 (TRIP6); Mouse Thyroid Receptor-Interacting Protein 6 (TRIP6) ELISA Kit; TRIP6; tcag7.1300; MGC10556; MGC10558; MGC29959; MGC3837; MGC4423; OIP1; ZRP-1; OPA-interacting protein 1; thyroid receptor-interacting protein 6; zyxin related protein 1; TRIP6 elisa kit
Reactivity
Mouse
Specificity
Mouse Thyroid receptor-interacting protein 6 (TRIP6) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TRIP6. No significant cross-reactivity or interference between Mouse TRIP6 and analogues was observed.
Sequence Length
480
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Thyroid receptor-interacting protein 6 (TRIP6) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Thyroid receptor-interacting protein 6 (TRIP6) Standard Curve.)
Related Product Information for TRIP6 elisa kit
Description: This Mouse Thyroid receptor-interacting protein 6 (TRIP6) ELISA Kit employs a two-site sandwich ELISA to quantitate TRIP6 in samples. An antibody specific for TRIP6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRIP6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRIP6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRIP6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: tRNA-isopentenyltransferase (tRNA-IPT) catalyses the addition of N6-isopentenyladenosine (i6A) on residue 37 of tRNA molecules that bind codons starting with uridine. The human tRNA-isopentenyltransferase (TRIT1) gene encodes a complex pattern of mRNA variants through alternative splicing in both normal and tumor lung tissue and that the nonsense suppressor activity of tRNA-IPT is maintained only in the full-length mRNA isoform, as revealed by gene complementation in yeast. Expression of the full-length transcript was down-regulated 6-14-fold in lung adenocarcinomas as compared to normal lung tissue. A549 lung cancer cells transfected to express the functional TRIT1 gene formed significantly smaller colonies with reduced scattering on the edges and had only limited ability to induce tumors in nude mice.
Background: tRNA-isopentenyltransferase (tRNA-IPT) catalyses the addition of N6-isopentenyladenosine (i6A) on residue 37 of tRNA molecules that bind codons starting with uridine. The human tRNA-isopentenyltransferase (TRIT1) gene encodes a complex pattern of mRNA variants through alternative splicing in both normal and tumor lung tissue and that the nonsense suppressor activity of tRNA-IPT is maintained only in the full-length mRNA isoform, as revealed by gene complementation in yeast. Expression of the full-length transcript was down-regulated 6-14-fold in lung adenocarcinomas as compared to normal lung tissue. A549 lung cancer cells transfected to express the functional TRIT1 gene formed significantly smaller colonies with reduced scattering on the edges and had only limited ability to induce tumors in nude mice.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
thyroid receptor-interacting protein 6
NCBI Official Synonym Full Names
thyroid hormone receptor interactor 6
NCBI Official Symbol
Trip6
NCBI Protein Information
thyroid receptor-interacting protein 6
UniProt Protein Name
Thyroid receptor-interacting protein 6
Protein Family
UniProt Gene Name
Trip6
UniProt Synonym Gene Names
Zrp1; TR-interacting protein 6; TRIP-6; ZRP-1
Uniprot Description
Relays signals from the cell surface to the nucleus to weaken adherens junction and promote actin cytoskeleton reorganization and cell invasiveness. Involved in lysophosphatidic acid-induced cell adhesion and migration. Acts as a transcriptional coactivator for NF-kappa-B and JUN, and mediates the transrepression of these transcription factors induced by glucocorticoid receptor ().
Research Articles on TRIP6
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Product Notes
The Mouse TRIP6 trip6 (Catalog #AAA9716667) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716667 ELISA Kit recognizes Mouse TRIP6. It is sometimes possible for the material contained within the vial of "Thyroid Receptor-Interacting Protein 6 (TRIP6), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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