Typical Testing Data/Standard Curve (for reference only)
Mouse Thy1 Cell Surface Antigen (Thy1) ELISA Kit | Thy1 elisa kit
Mouse Thy1 Cell Surface Antigen (Thy1) ELISA Kit
Gene Names
THY1; CD90; CDw90
Reactivity
Mouse
Synonyms
Thy1 Cell Surface Antigen (Thy1); Mouse Thy1 Cell Surface Antigen (Thy1) ELISA Kit; CD90; CDw90; THY mocyte differentiation antigen 1; Thy1 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Thy1 Cell Surface Antigen (Thy1).
No significant cross-reactivity or interference between Thy1 Cell Surface Antigen (Thy1) and analogues was observed.
No significant cross-reactivity or interference between Thy1 Cell Surface Antigen (Thy1) and analogues was observed.
Sequence Length
161
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Urine, Tissue Homogenates, Cell Lysates and Other Biological Fluids
Detection Range
12.50-800pg/mL
Sensitivity
< 5.7pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Thy1 Cell Surface Antigen (Thy1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Thy1 Cell Surface Antigen (Thy1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Thy1 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thy1 Cell Surface Antigen (Thy1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thy1 Cell Surface Antigen (Thy1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thy1 Cell Surface Antigen (Thy1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thy1 Cell Surface Antigen (Thy1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
17,935 Da
NCBI Official Full Name
Thy-1 cell surface antigen, isoform CRA_a
NCBI Official Synonym Full Names
Thy-1 cell surface antigen
NCBI Official Symbol
THY1
NCBI Official Synonym Symbols
CD90; CDw90
NCBI Protein Information
thy-1 membrane glycoprotein
UniProt Protein Name
Thy-1 membrane glycoprotein
Protein Family
UniProt Gene Name
THY1
NCBI Description
This gene encodes a cell surface glycoprotein and member of the immunoglobulin superfamily of proteins. The encoded protein is involved in cell adhesion and cell communication in numerous cell types, but particularly in cells of the immune and nervous systems. The encoded protein is widely used as a marker for hematopoietic stem cells. This gene may function as a tumor suppressor in nasopharyngeal carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
Uniprot Description
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
Research Articles on Thy1
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Product Notes
The Mouse Thy1 thy1 (Catalog #AAA2703273) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2703273 ELISA Kit recognizes Mouse Thy1. It is sometimes possible for the material contained within the vial of "Thy1 Cell Surface Antigen (Thy1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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