Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse TAR DNA-binding protein 43 (TARDBP) Standard Curve.)
Mouse TAR DNA-Binding Protein 43 (TARDBP) ELISA Kit | TARDBP elisa kit
Mouse TAR DNA-Binding Protein 43 (TARDBP) ELISA Kit
Gene Names
Tardbp; Tdp43; C85084; TDP-43; 1190002A23Rik
Reactivity
Mouse
Synonyms
TAR DNA-Binding Protein 43 (TARDBP); Mouse TAR DNA-Binding Protein 43 (TARDBP) ELISA Kit; TARDBP; RP4-635E18.2; ALS10; TDP-43; TAR DNA-binding protein-43; TARDBP elisa kit
Reactivity
Mouse
Specificity
Mouse TAR DNA-binding protein 43 (TARDBP) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TARDBP. No significant cross-reactivity or interference between Mouse TARDBP and analogues was observed.
Sequence Length
414
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse TAR DNA-binding protein 43 (TARDBP) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse TAR DNA-binding protein 43 (TARDBP) Standard Curve.)
Related Product Information for TARDBP elisa kit
Description: This Mouse TAR DNA-binding protein 43 (TARDBP) ELISA Kit employs a two-site sandwich ELISA to quantitate TARDBP in samples. An antibody specific for TARDBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TARDBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TARDBP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TARDBP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by TARDBP is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.
Background: HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by TARDBP is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
TAR DNA-binding protein 43 isoform 1
NCBI Official Synonym Full Names
TAR DNA binding protein
NCBI Official Symbol
Tardbp
NCBI Official Synonym Symbols
Tdp43; C85084; TDP-43; 1190002A23Rik
NCBI Protein Information
TAR DNA-binding protein 43
UniProt Protein Name
TAR DNA-binding protein 43
Protein Family
UniProt Gene Name
Tardbp
UniProt Synonym Gene Names
Tdp43; TDP-43
UniProt Entry Name
TADBP_MOUSE
Research Articles on TARDBP
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Product Notes
The Mouse TARDBP tardbp (Catalog #AAA9716956) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716956 ELISA Kit recognizes Mouse TARDBP. It is sometimes possible for the material contained within the vial of "TAR DNA-Binding Protein 43 (TARDBP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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