Typical Testing Data/Standard Curve (for reference only)
Mouse Syndecan-1/SDC1 ELISA Kit | Sdc1 elisa kit
Mouse Syndecan-1/SDC1 PicoKine ELISA Kit
Gene Names
Sdc1; Sstn; Synd; CD138; Synd1; syn-1; AA408134; AA409076
Reactivity
Mouse
Synonyms
Syndecan-1/SDC1; Mouse Syndecan-1/SDC1 PicoKine ELISA Kit; CD138; P18827; SDC1; SYND1; Synd-1; Synd1; syndecan1; Syndecan-1; Sdc1 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse Sdc1
Sequence Length
311
Samples
Cell culture supernatants, cell lysates, serum and plasma (heparin, edta)
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<10 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Sdc1 elisa kit
Principle of the Assay: The Picokine™ Mouse Sdc1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Sdc1 with a 96-well strip plate that is pre-coated with antibody specific for Sdc1. The detection antibody is a biotinylated antibody specific for Sdc1. The capture antibody is a monoclonal antibody from rat and the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Mouse Sdc1 with immunogen: Expression system for standard: NS0; Immunogen sequence: Q18-E252. The kit is analytically validated with ready-to-use reagents. To measure Mouse Sdc1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Sdc1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Sdc1 in the sample.
Background/Introduction: Syndecan 1, also known as SYND1 or CD138, is a protein which in humans is encoded by the SDC1 gene. The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. This gene is mapped to 2p24.1. The syndecans mediate cell binding, cell signaling, cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan 1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. What's more, Syndecan-1 is also a sponge for growth factors, with binding largely via heparan sulfate chains.
Background/Introduction: Syndecan 1, also known as SYND1 or CD138, is a protein which in humans is encoded by the SDC1 gene. The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. This gene is mapped to 2p24.1. The syndecans mediate cell binding, cell signaling, cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan 1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. What's more, Syndecan-1 is also a sponge for growth factors, with binding largely via heparan sulfate chains.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,235 Da
NCBI Official Full Name
syndecan-1
NCBI Official Synonym Full Names
syndecan 1
NCBI Official Symbol
Sdc1
NCBI Official Synonym Symbols
Sstn; Synd; CD138; Synd1; syn-1; AA408134; AA409076
NCBI Protein Information
syndecan-1
UniProt Protein Name
Syndecan-1
Protein Family
UniProt Gene Name
Sdc1
UniProt Synonym Gene Names
Synd-1; Synd1; SYND1
Uniprot Description
syndecan-1: Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Belongs to the syndecan proteoglycan family.
Protein type: Extracellular matrix; Membrane protein, integral; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 12 A1.1|12 3.94 cM
Cellular Component: cell surface; cytoplasm; external side of plasma membrane; focal adhesion; protein complex
Molecular Function: glycoprotein binding; protein C-terminus binding
Biological Process: cell migration; inflammatory response; myoblast development; Wnt receptor signaling pathway through beta-catenin
Research Articles on Sdc1
Similar Products
Product Notes
The Mouse Sdc1 sdc1 (Catalog #AAA178742) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA178742 ELISA Kit recognizes Mouse Sdc1. It is sometimes possible for the material contained within the vial of "Syndecan-1/SDC1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
