Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Synaptotagmin-12 (SYT12) Standard Curve.)
Mouse Synaptotagmin-12 (SYT12) ELISA Kit | SYT12 elisa kit
Mouse Synaptotagmin-12 (SYT12) ELISA Kit
Reactivity
Mouse
Synonyms
Synaptotagmin-12 (SYT12); Mouse Synaptotagmin-12 (SYT12) ELISA Kit; SYT12; SRG1; SYT11; SYT12 elisa kit
Reactivity
Mouse
Specificity
Mouse Synaptotagmin-12 (SYT12) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SYT12. No significant cross-reactivity or interference between Mouse SYT12 and analogues was observed.
Sequence Length
421
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Synaptotagmin-12 (SYT12) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Synaptotagmin-12 (SYT12) Standard Curve.)
Related Product Information for SYT12 elisa kit
Description: This Mouse Synaptotagmin-12 (SYT12) ELISA Kit employs a two-site sandwich ELISA to quantitate SYT12 in samples. An antibody specific for SYT12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SYT12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYT12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYT12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SYT12 is a member of the synaptotagmin gene family and encodes a protein similar to other family members that mediate calcium-dependent regulation of membrane trafficking in synaptic transmission. Studies of the orthologous gene in rat have shown that the encoded protein selectively modulates spontaneous synaptic-vesicle exocytosis and may also be involved in regulating calcium independent secretion in nonneuronal cells. Alternative splicing results in multiple transcript variants. SYT12 has previously been referred to as synaptotagmin XI but has been renamed synaptotagmin XII to be standard with mouse and rat official nomenclature.
Background: SYT12 is a member of the synaptotagmin gene family and encodes a protein similar to other family members that mediate calcium-dependent regulation of membrane trafficking in synaptic transmission. Studies of the orthologous gene in rat have shown that the encoded protein selectively modulates spontaneous synaptic-vesicle exocytosis and may also be involved in regulating calcium independent secretion in nonneuronal cells. Alternative splicing results in multiple transcript variants. SYT12 has previously been referred to as synaptotagmin XI but has been renamed synaptotagmin XII to be standard with mouse and rat official nomenclature.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
synaptotagmin-12
NCBI Official Synonym Full Names
synaptotagmin XII
NCBI Official Symbol
Syt12
NCBI Protein Information
synaptotagmin-12
UniProt Protein Name
Synaptotagmin-12
Protein Family
UniProt Gene Name
Syt12
UniProt Synonym Gene Names
SytXII
UniProt Entry Name
SYT12_MOUSE
Uniprot Description
SYT12: May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Belongs to the synaptotagmin family.
Protein type: Membrane protein, integral; Vesicle
Cellular Component: membrane; plasma membrane; integral to membrane; synapse; cytoplasmic vesicle; cell junction
Molecular Function: clathrin binding; syntaxin binding; calcium-dependent phospholipid binding; calcium ion binding
Biological Process: vesicle fusion; synaptic vesicle exocytosis; calcium ion-independent exocytosis of neurotransmitter; calcium ion-dependent exocytosis of neurotransmitter; regulation of calcium ion-dependent exocytosis
Research Articles on SYT12
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Product Notes
The Mouse SYT12 syt12 (Catalog #AAA9716906) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716906 ELISA Kit recognizes Mouse SYT12. It is sometimes possible for the material contained within the vial of "Synaptotagmin-12 (SYT12), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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