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Typical Testing Data/Standard Curve (for reference only)

Mouse Sulfite Oxidase (SUOX) ELISA Kit | SUOX elisa kit

Mouse Sulfite Oxidase (SUOX) ELISA Kit

Reactivity
Mouse
Synonyms
Sulfite Oxidase (SUOX); Mouse Sulfite Oxidase (SUOX) ELISA Kit; SUOX elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Sulfite Oxidase (SUOX).
No significant cross-reactivity or interference between Sulfite Oxidase (SUOX) and analogues was observed.
Sequence Length
545
Assay Type
Double-antibody Sandwich
Samples
Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.058ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Sulfite Oxidase (SUOX) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Sulfite Oxidase (SUOX) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SUOX elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sulfite Oxidase (SUOX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sulfite Oxidase (SUOX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sulfite Oxidase (SUOX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sulfite Oxidase (SUOX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
60,283 Da
NCBI Official Full Name
sulfite oxidase
NCBI Official Synonym Full Names
sulfite oxidase
NCBI Official Symbol
SUOX
NCBI Protein Information
sulfite oxidase, mitochondrial
UniProt Protein Name
Sulfite oxidase, mitochondrial
Protein Family
UniProt Gene Name
SUOX

NCBI Description

Sulfite oxidase is a homodimeric protein localized to the intermembrane space of mitochondria. Each subunit contains a heme domain and a molybdopterin-binding domain. The enzyme catalyzes the oxidation of sulfite to sulfate, the final reaction in the oxidative degradation of the sulfur amino acids cysteine and methionine. Sulfite oxidase deficiency results in neurological abnormalities which are often fatal at an early age. Alternative splicing results in multiple transcript variants encoding identical proteins. [provided by RefSeq, Jul 2008]

Research Articles on SUOX

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Product Notes

The Mouse SUOX suox (Catalog #AAA2706312) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2706312 ELISA Kit recognizes Mouse SUOX. It is sometimes possible for the material contained within the vial of "Sulfite Oxidase (SUOX), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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