Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial (SDHD) Standard Curve.)
Mouse Succinate Dehydrogenase [Ubiquinone] Cytochrome B Small Subunit, Mitochondrial (SDHD) ELISA Kit | SDHD elisa kit
Mouse Succinate Dehydrogenase [Ubiquinone] Cytochrome B Small Subunit, Mitochondrial (SDHD) ELISA Kit
Gene Names
Sdhd; C78570; AVLL5809; PRO19626; 3110001M13Rik
Reactivity
Mouse
Synonyms
Succinate Dehydrogenase [Ubiquinone] Cytochrome B Small Subunit; Mitochondrial (SDHD); Mouse Succinate Dehydrogenase [Ubiquinone] Cytochrome B Small Subunit; Mitochondrial (SDHD) ELISA Kit; SDHD; CBT1; PGL; PGL1; SDH4; succinate dehydrogenase complex; subunit D; succinate dehydrogenase ubiquinone cytochrome B small subunit; SDHD elisa kit
Reactivity
Mouse
Specificity
Mouse Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial (SDHD) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SDHD. No significant cross-reactivity or interference between Mouse SDHD and analogues was observed.
Sequence Length
159
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial (SDHD) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial (SDHD) Standard Curve.)
Related Product Information for SDHD elisa kit
Description: This Mouse Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial (SDHD) ELISA Kit employs a two-site sandwich ELISA to quantitate SDHD in samples. An antibody specific for SDHD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SDHD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SDHD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDHD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SDHD, which stands for succinate dehydrogenase complex subunit D, is one of the two transmembrane subunits of the four-subunit succinate dehydrogenase protein complex that resides in the inner mitochondrial membrane. It also refers to the gene that codes for this protein. Germline mutations in SDHD were first linked to hereditary paraganglioma in 2000. Since then, it has been shown that mutations in SDHB and to a lesser degree SDHC can cause paranglioma as well familial pheochromocytoma. Notably, the tumor spectrum is different for the different mutations. SDHB mutations often lead to metastatic disease that is extra-adrenal, while SDHD mutation related tumors are more typically benign, originating in the head and neck.
Background: SDHD, which stands for succinate dehydrogenase complex subunit D, is one of the two transmembrane subunits of the four-subunit succinate dehydrogenase protein complex that resides in the inner mitochondrial membrane. It also refers to the gene that codes for this protein. Germline mutations in SDHD were first linked to hereditary paraganglioma in 2000. Since then, it has been shown that mutations in SDHB and to a lesser degree SDHC can cause paranglioma as well familial pheochromocytoma. Notably, the tumor spectrum is different for the different mutations. SDHB mutations often lead to metastatic disease that is extra-adrenal, while SDHD mutation related tumors are more typically benign, originating in the head and neck.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
succinate dehydrogenase
NCBI Official Synonym Full Names
succinate dehydrogenase complex, subunit D, integral membrane protein
NCBI Official Symbol
Sdhd
NCBI Official Synonym Symbols
C78570; AVLL5809; PRO19626; 3110001M13Rik
NCBI Protein Information
succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial
UniProt Protein Name
Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial
Protein Family
UniProt Gene Name
Sdhd
UniProt Synonym Gene Names
CybS
Uniprot Description
Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
Research Articles on SDHD
Similar Products
Product Notes
The Mouse SDHD sdhd (Catalog #AAA9717075) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717075 ELISA Kit recognizes Mouse SDHD. It is sometimes possible for the material contained within the vial of "Succinate Dehydrogenase [Ubiquinone] Cytochrome B Small Subunit, Mitochondrial (SDHD), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
