Typical Testing Data/Standard Curve (for reference only)
Mouse Stathmin-2 (STMN2) ELISA Kit | STMN2 elisa kit
Mouse Stathmin-2 (STMN2) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: STMN-2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-STMN-2 antibody and an STMN-2-HRP conjugate. The assay sample and buffer are incubated together with STMN-2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the STMN-2 concentration since STMN-2 from samples and STMN-2-HRP conjugate compete for the anti-STMN-2 antibody binding site. Since the number of sites is limited, as more sites are occupied by STMN-2 from the sample, fewer sites are left to bind STMN-2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The STMN-2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
regulatory phosphoprotein; involved in promoting microtubule depolymerization and maintenance of long-term synaptic plasticity [RGD, Feb 2006]
Uniprot Description
STMN2: a cytoskeletal protein that may play a role in neuronal differentiation, and in modulating membrane interaction with the cytoskeleton during neurite outgrowth. Associated with punctate structures in the perinuclear cytoplasm, axons, and growth cones of developing neurons. Neuron specific.
Protein type: Cytoskeletal
Cellular Component: Golgi apparatus; neuron projection; growth cone; cell soma; membrane; axon; perinuclear region of cytoplasm; lamellipodium; cytoplasm; endosome; vesicle
Molecular Function: tubulin binding; protein binding; calcium-dependent protein binding
Biological Process: positive regulation of microtubule depolymerization; negative regulation of microtubule depolymerization; negative regulation of microtubule polymerization; microtubule depolymerization; neurite development; regulation of cytoskeleton organization and biogenesis
Research Articles on STMN2
Similar Products
Product Notes
The Mouse STMN2 stmn2 (Catalog #AAA7223765) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7223765 ELISA Kit recognizes Mouse STMN2. It is sometimes possible for the material contained within the vial of "Stathmin-2 (STMN2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
