Mouse Solute Carrier Family 30, Member 5 (SLC30A5) ELISA Kit | SLC30A5 elisa kit

Mouse Solute Carrier Family 30, Member 5 (SLC30A5) ELISA Kit

Cat. Num. AAA2706176

• Gene Names: SLC30A5; ZNT5; ZTL1; ZNTL1; ZnT-5
• Reactivity: Mouse
• Synonyms: Solute Carrier Family 30; Member 5 (SLC30A5); Mouse Solute Carrier Family 30; Member 5 (SLC30A5) ELISA Kit; ZTL1; ZnT-5; ZNT5; ZNTL1; Zinc Transporter 5; ZnT-like transporter 1; SLC30A5 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Solute Carrier Family 30, Member 5 (SLC30A5).; No significant cross-reactivity or interference between Solute Carrier Family 30, Member 5 (SLC30A5) and analogues was observed.
• Sequence Length: 77

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.108ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Solute Carrier Family 30, Member 5 (SLC30A5) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Solute Carrier Family 30, Member 5 (SLC30A5) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SLC30A5 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Solute Carrier Family 30, Member 5 (SLC30A5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Solute Carrier Family 30, Member 5 (SLC30A5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Solute Carrier Family 30, Member 5 (SLC30A5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Solute Carrier Family 30, Member 5 (SLC30A5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119571695
• NCBI GeneID: 64924
• UniProt Accession #: Q8TAD4; Q6UX54; Q7L4M4; Q8TDG3; Q9BVY8; Q9H9H1; B7ZM89
• Molecular Weight: 8,216 Da
• NCBI Official Full Name: solute carrier family 30 (zinc transporter), member 5, isoform CRA_d
• NCBI Official Synonym Full Names: solute carrier family 30 member 5
• NCBI Official Symbol: SLC30A5
• NCBI Official Synonym Symbols: ZNT5; ZTL1; ZNTL1; ZnT-5
• NCBI Protein Information: zinc transporter 5
• UniProt Protein Name: Zinc transporter 5
• Protein Family: Zinc transporter
• UniProt Gene Name: SLC30A5
• UniProt Synonym Gene Names: ZNT5; ZNTL1; ZTL1; ZnT-5; hZTL1

NCBI Description

This gene encodes a member of the SLC30A/ZnT family of zinc transporter proteins. ZnT proteins mediate both cellular zinc efflux and zinc sequestration into membrane-bound organelles. The encoded protein plays a role in the early secretory pathway as a heterodimer with zinc transporter 6, and may also regulate zinc sequestration into secretory granules of pancreatic beta cells. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 19. [provided by RefSeq, Oct 2011]

Uniprot Description

Functions as a zinc transporter. May be a transporter of zinc into beta cells in order to form insulin crystals. Partly regulates cellular zinc homeostasis. Required with ZNT7 for the activation of zinc-requiring enzymes, alkaline phosphatases (ALPs). Transports zinc into the lumens of the Golgi apparatus and vesicular compartments where ALPs locate, thus, converting apoALPs to holoALPs. Required with ZNT6 and ZNT7 for the activation of TNAP.