Mouse Soluble CD14 ELISA Kit | Cd14 elisa kit

Mouse Soluble CD14 ELISA Kit

Cat. Num. AAA3807351

• Reactivity: Mouse
• Synonyms: Soluble CD14; Mouse Soluble CD14 ELISA Kit; Cd14 elisa kit

• Reactivity: Mouse
• Sequence Length: 366

• Samples: Serum, plasma, cell culture supernate and other biological fluids
• Assay Type: Sandwich
• Detection Range: 112.5ng/ml-1800ng/ml
• Sensitivity: 10 ng/ml

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Cd14 elisa kit

Intended Use: The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofSOLUBLE CD14in the sample, This SOLUBLE CD14ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusSOLUBLE CD14concentration. The concentrationofSOLUBLE CD14in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6753332
• NCBI GeneID: 12475
• NCBI Accession #: NP_033971.1
• NCBI GenBank Nucleotide #: NP_033971.1
• UniProt Accession #: P10810
• NCBI Official Full Name: monocyte differentiation antigen CD14
• NCBI Official Synonym Full Names: CD14 antigen
• NCBI Official Symbol: Cd14
• NCBI Protein Information: monocyte differentiation antigen CD14
• UniProt Protein Name: Monocyte differentiation antigen CD14
• Protein Family: Monocyte differentiation antigen
• UniProt Gene Name: Cd14
• UniProt Entry Name: CD14_MOUSE

NCBI Description

This gene encodes a protein that plays an important role in the innate immune response and is expressed in monocyte/macrophage cells. This gene product acts as a co-receptor that binds several microbial and fungal molecules, including lipopolysaccharides (LPS). This proteins LPS-binding activity is enhanced by the LPS binding protein (LBP) to allow binding to the TLR4-MD-2 co-receptor complex. The product of this gene is found in two forms, either as a soluble protein or attached to the cell surface by a glycosylphosphatidylinositol anchor. [provided by RefSeq, Jul 2014]

Uniprot Description

CD14: Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

Protein type: Membrane protein, GPI anchor

Cellular Component: anchored to external side of plasma membrane; cell surface; external side of plasma membrane; extracellular space; Golgi apparatus; lipid raft; lipopolysaccharide receptor complex; plasma membrane

Molecular Function: lipopolysaccharide binding

Biological Process: inflammatory response; lipopolysaccharide-mediated signaling pathway; positive regulation of cytokine secretion; positive regulation of endocytosis; positive regulation of interferon type I production; positive regulation of interferon-gamma production; positive regulation of tumor necrosis factor production; receptor-mediated endocytosis; response to bacterium; response to molecule of bacterial origin; toll-like receptor 4 signaling pathway