Western Blot (WB)
(Anti-PDK2 antibody, MBS176091, Western blottingLane 1: Rat Heart Tissue LysateLane 2: HELA Cell LysateLane 3: SW620 Cell LysateLane 4: MCF-7 Cell Lysate )
Rabbit PDK2 Polyclonal Antibody | anti-PDK2 antibody
Anti-PDK2 antibody
Western Blot (WB)
(Anti-PDK2 antibody, MBS176091, Western blottingLane 1: Rat Heart Tissue LysateLane 2: HELA Cell LysateLane 3: SW620 Cell LysateLane 4: MCF-7 Cell Lysate )
Western Blot (WB)
(Anti-PDK2 antibody, MBS176091, Western blottingLane 1: Rat Heart Tissue LysateLane 2: HELA Cell LysateLane 3: SW620 Cell LysateLane 4: MCF-7 Cell Lysate )
Immunohistochemistry (IHC)
(Anti-PDK2 antibody, MBS176091, IHC(P)IHC(P): Human Mammary Cancer Tissue )
Immunohistochemistry (IHC)
(Anti-PDK2 antibody, MBS176091, IHC(P)IHC(P): Human Mammary Cancer Tissue )
Immunohistochemistry (IHC)
(Anti-PDK2 antibody, MBS176091, IHC(P)IHC(P): Rat Brain Tissue )
Immunohistochemistry (IHC)
(Anti-PDK2 antibody, MBS176091, IHC(P)IHC(P): Rat Brain Tissue )
Immunocytochemistry (ICC)
(Anti-PDK2 antibody, MBS176091, ICCICC: HELA Cell )
Immunocytochemistry (ICC)
(Anti-PDK2 antibody, MBS176091, ICCICC: HELA Cell )
Background: PDK2(Pyruvate Dehydrogenase Kinase Isoenzyme 2), is an enzyme that in humans is encoded by the PDK2 gene. To find human PDKs, Gudi et al.(1995) used oligonucleotide primers to screen a human liver cDNA library by PCR. The human PDK2 gene encodes a protein with a predicted molecular mass of 46,181 Da that shares 96% identity with that of the rat PDK2 gene. Northern blot analysis showed that the PDK2 message was found in many tissues, with the highest level in heart and skeletal muscle, intermediate levels in brain, kidney, pancreas and liver, and low levels in placenta and lung.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]
Uniprot Description
PDHK2: an atypical protein kinase associated with the mitochondrial matrix. The PDHKs play crucial roles in switching metabolic flux from oxidative phosphorylation towards glycolysis. PDHK2 is expressed in all tissues. Contains a HATPase_c catalytic domain, found in several ATP-binding proteins including protein histidine kinases (PHKs), PHDKs, DNA gyrase B, topoisomerases, heat shock proteins, and DNA mismatch repair proteins. PDHK regulates glucose oxidation through inhibitory phosphorylation of the E1 alpha subunit of the mitochondrial pyruvate dehydrogenase complex (PDHC) at any one of 3 inhibitory serine residues. Inhibitory sites 1, 2, and 3 correspond to S293, S300, and S232 in human PDHA1, respectively. Four PDHK isoenzymes have been described, each with different site specificity: all four phosphorylate sites 1 and 2 but at different rates; for site 1 PDHK2 >PDHK4 >PDHK1 >PDHK3; for site 2, PDHK3> PDHK4 > PDHK2 > PDHK1. Only PDHK1 phosphorylates site 3. PDHKs are recruited to the PDHC by binding to a lipoyl group covalently attached to the inner lipoyl domain of the E2 component. PDHA1 deficiency is the most common enzyme defect in patients with primary lactic acidosis. Suppression of PDH by PDHK inhibits the conversion of pyruvate to acetyl-CoA, attenuates mitochondrial respiration, and may contribute to the increased lactate production observed in many tumors. The PDH pathway is repressed in a majority of non-small cell lung carcinomas. Inhibited by AZD7545, dichloroacetate (DCA), and radicicol. Radicicol inhibits kinase activity by binding directly to the ATP-binding pocket of PDHK, similar to HSP90 from the same ATPase/kinase superfamily.
Protein type: Kinase, protein; EC 2.7.11.2; Mitochondrial; Protein kinase, atypical; ATYPICAL group; PDHK family; PDHK subfamily
Chromosomal Location of Human Ortholog: 17q21.33
Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; cytoplasm; mitochondrial pyruvate dehydrogenase complex
Molecular Function: protein homodimerization activity; pyruvate dehydrogenase (acetyl-transferring) kinase activity; ATP binding; protein kinase activity
Biological Process: cellular metabolic process; insulin receptor signaling pathway; glucose metabolic process; regulation of pH; cellular response to nutrient; regulation of gluconeogenesis; regulation of acetyl-CoA biosynthetic process from pyruvate; glucose homeostasis; pyruvate metabolic process; protein amino acid phosphorylation
Research Articles on PDK2
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Product Notes
The PDK2 pdk2 (Catalog #AAA176091) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PDK2 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PDK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC). Researchers should empirically determine the suitability of the PDK2 pdk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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