Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Serine palmitoyltransferase 2 (SPTLC2) Standard Curve.)
Mouse Serine Palmitoyltransferase 2 (SPTLC2) ELISA Kit | SPTLC2 elisa kit
Mouse Serine Palmitoyltransferase 2 (SPTLC2) ELISA Kit
Gene Names
Sptlc2; LCB2; Spt2; LCB2a
Reactivity
Mouse
Synonyms
Serine Palmitoyltransferase 2 (SPTLC2); Mouse Serine Palmitoyltransferase 2 (SPTLC2) ELISA Kit; SPTLC2; KIAA0526; LCB2; SPT2; serine palmitoyltransferase; subunit; SPTLC2 elisa kit
Reactivity
Mouse
Specificity
Mouse Serine palmitoyltransferase 2 (SPTLC2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SPTLC2. No significant cross-reactivity or interference between Mouse SPTLC2 and analogues was observed.
Sequence Length
560
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Serine palmitoyltransferase 2 (SPTLC2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Serine palmitoyltransferase 2 (SPTLC2) Standard Curve.)
Related Product Information for SPTLC2 elisa kit
Description: This Mouse Serine palmitoyltransferase 2 (SPTLC2) ELISA Kit employs a two-site sandwich ELISA to quantitate SPTLC2 in samples. An antibody specific for SPTLC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPTLC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPTLC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPTLC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Serine palmitoyltransferase (SPT; EC 2.3.1.50) is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine. SPTLC2encodes a long chain base subunit of serine palmitoyltransferase. Serine palmitoyltransferase, which consists of two different subunits, is the initial enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal 5'-phosphate dependent condensation of L-serine and palmitoyl CoA to 3-oxosphinganine. Mutations in this gene were identified in patients with hereditary sensory neuropathy type I. Alternatively spliced variants encoding different isoforms have been identified.The LCB2 cDNA encodes a protein of 562 amino acids that is more than 90% identical to the mouse protein.
Background: Serine palmitoyltransferase (SPT; EC 2.3.1.50) is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine. SPTLC2encodes a long chain base subunit of serine palmitoyltransferase. Serine palmitoyltransferase, which consists of two different subunits, is the initial enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal 5'-phosphate dependent condensation of L-serine and palmitoyl CoA to 3-oxosphinganine. Mutations in this gene were identified in patients with hereditary sensory neuropathy type I. Alternatively spliced variants encoding different isoforms have been identified.The LCB2 cDNA encodes a protein of 562 amino acids that is more than 90% identical to the mouse protein.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
serine palmitoyltransferase 2
NCBI Official Synonym Full Names
serine palmitoyltransferase, long chain base subunit 2
NCBI Official Symbol
Sptlc2
NCBI Official Synonym Symbols
LCB2; Spt2; LCB2a
NCBI Protein Information
serine palmitoyltransferase 2
UniProt Protein Name
Serine palmitoyltransferase 2
Protein Family
UniProt Gene Name
Sptlc2
UniProt Synonym Gene Names
Lcb2; LCB 2; LCB2a; SPT 2
NCBI Description
This gene encodes a long chain base subunit of serine palmitoyltransferase. The enzyme, serine palmitoyltransferase, consists of two different subunits, and is the key enzyme in sphingolipid biosynthesis. It catalyzes the pyridoxal-5-prime-phosphate-dependent condensation of L-serine and palmitoyl-CoA to 3-oxosphinganine. A mutant allele of this gene in mice is used as a model for the human disease 'Susceptibilty to Psoriasis 1'. Mutations in the human gene are associated with hereditary sensory neuropathy type I. [provided by RefSeq, Sep 2015]
Uniprot Description
Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC2-SPTSSB complex displays a preference for C18-CoA substrate ().
Research Articles on SPTLC2
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Product Notes
The Mouse SPTLC2 sptlc2 (Catalog #AAA9716860) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716860 ELISA Kit recognizes Mouse SPTLC2. It is sometimes possible for the material contained within the vial of "Serine Palmitoyltransferase 2 (SPTLC2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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