Mouse Rho-associated coiled-coil containing protein kinase 1 (Rock-1) ELISA Kit | Rock-1 elisa kit

Mouse Rho-associated coiled-coil containing protein kinase 1 (Rock-1) ELISA Kit

Cat. Num. AAA1601258

• Gene Names: ROCK1; ROCK-I; P160ROCK
• Reactivity: Mouse
• Synonyms: Rho-associated coiled-coil containing protein kinase 1 (Rock-1); Mouse Rho-associated coiled-coil containing protein kinase 1 (Rock-1) ELISA Kit; Rock-1 elisa kit

• Reactivity: Mouse
• Sequence Length: 1354

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.05ng/ml - 10ng/ml
• Sensitivity: 0.023ng/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Rock-1 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse Rho-associated Coiled-coil Containing Protein Kinase 1 (also known as Rock-1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse Rock-1 antibody. Rock-1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse Rock-1 Antibody is added and binds to Rock-1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated Rock-1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse Rock-1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 119621524
• NCBI GeneID: 6093
• Molecular Weight: 158,175 Da
• NCBI Official Full Name: Rho-associated, coiled-coil containing protein kinase 1
• NCBI Official Synonym Full Names: Rho associated coiled-coil containing protein kinase 1
• NCBI Official Symbol: ROCK1
• NCBI Official Synonym Symbols: ROCK-I; P160ROCK
• NCBI Protein Information: rho-associated protein kinase 1
• UniProt Protein Name: Rho-associated protein kinase 1
• UniProt Gene Name: ROCK1
• UniProt Synonym Gene Names: ROCK-I; p160ROCK

NCBI Description

This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18. [provided by RefSeq, Aug 2015]

Uniprot Description

Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization.