Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Regulator of G-protein signaling 5 (RGS5) Standard Curve.)
Mouse Regulator Of G-Protein Signaling 5 (RGS5) ELISA Kit | RGS5 elisa kit
Mouse Regulator Of G-Protein Signaling 5 (RGS5) ELISA Kit
Gene Names
Rgs5; 1110070A02Rik
Reactivity
Mouse
Synonyms
Regulator Of G-Protein Signaling 5 (RGS5); Mouse Regulator Of G-Protein Signaling 5 (RGS5) ELISA Kit; RGS5; RP11-430G6.2; MST092; MST106; MST129; MSTP032; MSTP092; MSTP106; MSTP129; Regulator of G protein signaling-5; regulator of G-protein signalling 5; RGS5 elisa kit
Reactivity
Mouse
Specificity
Mouse Regulator of G-protein signaling 5 (RGS5) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse RGS5. No significant cross-reactivity or interference between Mouse RGS5 and analogues was observed.
Sequence Length
181
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Regulator of G-protein signaling 5 (RGS5) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Regulator of G-protein signaling 5 (RGS5) Standard Curve.)
Related Product Information for RGS5 elisa kit
Description: This Mouse Regulator of G-protein signaling 5 (RGS5) ELISA Kit employs a two-site sandwich ELISA to quantitate RGS5 in samples. An antibody specific for RGS5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RGS5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RGS5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RGS5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The regulator of G protein signaling (RGS) proteins are signal transduction molecules that have structural homology to SST2 of Saccharomyces cerevisiae and EGL-10 of Caenorhabditis elegans. Multiple genes homologous to SST2 are present in higher eukaryotes. RGS proteins are involved in the regulation of heterotrimeric G proteins by acting as GTPase activators. The amino acid sequence deduced from the cDNA possessed all consensus motifs of the RGS domain and showed closest homology to mouse Rgs5, with which it was 90% identical, indicating that it represented human RGS5. The mRNA of the human gene is abundantly expressed in heart, lung, skeletal muscle, and small intestine, and at low levels in brain, placenta, liver, colon, and leukocytes.
Background: The regulator of G protein signaling (RGS) proteins are signal transduction molecules that have structural homology to SST2 of Saccharomyces cerevisiae and EGL-10 of Caenorhabditis elegans. Multiple genes homologous to SST2 are present in higher eukaryotes. RGS proteins are involved in the regulation of heterotrimeric G proteins by acting as GTPase activators. The amino acid sequence deduced from the cDNA possessed all consensus motifs of the RGS domain and showed closest homology to mouse Rgs5, with which it was 90% identical, indicating that it represented human RGS5. The mRNA of the human gene is abundantly expressed in heart, lung, skeletal muscle, and small intestine, and at low levels in brain, placenta, liver, colon, and leukocytes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
regulator of G-protein signaling 5 isoform 1
NCBI Official Synonym Full Names
regulator of G-protein signaling 5
NCBI Official Symbol
Rgs5
NCBI Official Synonym Symbols
1110070A02Rik
NCBI Protein Information
regulator of G-protein signaling 5
UniProt Protein Name
Regulator of G-protein signaling 5
Protein Family
UniProt Gene Name
Rgs5
UniProt Synonym Gene Names
RGS5
UniProt Entry Name
RGS5_MOUSE
Research Articles on RGS5
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Product Notes
The Mouse RGS5 rgs5 (Catalog #AAA9717112) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717112 ELISA Kit recognizes Mouse RGS5. It is sometimes possible for the material contained within the vial of "Regulator Of G-Protein Signaling 5 (RGS5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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