Mouse Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) ELISA Kit | RANTES elisa kit

Mouse Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) Mini Samples ELISA Kit

Cat. Num. AAA2708257

• Gene Names: Ccl5; SISd; Scya5; RANTES; TCP228; MuRantes
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES); Mouse Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) Mini Samples ELISA Kit; Regulated On Activation In Normal T-Cell Expressed And Secreted; CCL5; SCYA5; SISd; EoCP; Chemokine C-C-Motif Ligand 5; Eosinophil Chemotactic Cytokine; T-cell Specific Protein p288; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 5; RANTES elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES).; No significant cross-reactivity or interference between Mini Samples Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) and analogues was observed.
• Sequence Length: 91

• Applicable Applications for RANTES elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Mini Samples Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

NCBI and Uniprot Product Information

• NCBI GI #: 164698428
• NCBI GeneID: 20304
• NCBI Accession #: NP_038681.2
• NCBI GenBank Nucleotide #: NP_038681.2
• UniProt Accession #: P30882
• NCBI Official Full Name: C-C motif chemokine 5
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 5
• NCBI Official Symbol: Ccl5
• NCBI Official Synonym Symbols: SISd; Scya5; RANTES; TCP228; MuRantes
• NCBI Protein Information: C-C motif chemokine 5
• UniProt Protein Name: C-C motif chemokine 5
• UniProt Gene Name: Ccl5
• UniProt Synonym Gene Names: Scya5
• UniProt Entry Name: CCL5_MOUSE

Uniprot Description

CCL5: Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T- cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils. By mitogens. T-cell and macrophage specific. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Cell adhesion; Secreted, signal peptide; Chemokine

Cellular Component: extracellular space; cell; cytoplasm; extracellular region; intracellular

Molecular Function: heparin binding; protein homodimerization activity; protein self-association; cytokine activity; receptor signaling protein tyrosine kinase activator activity; phosphoinositide phospholipase C activity; protein kinase activity; CCR1 chemokine receptor binding; chemokine receptor binding; chemokine activity; chemokine receptor antagonist activity; CCR5 chemokine receptor binding; chemoattractant activity; phospholipase activator activity

Biological Process: positive regulation of cell adhesion; exocytosis; positive regulation of osteoclast differentiation; response to toxin; positive regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell migration; chemotaxis; protein amino acid phosphorylation; positive regulation of cell-cell adhesion mediated by integrin; pseudopodium formation; positive regulation of homotypic cell-cell adhesion; leukocyte adhesion; positive chemotaxis; cell-cell signaling; calcium ion transport; lipopolysaccharide-mediated signaling pathway; protein kinase B signaling cascade; positive regulation of T cell proliferation; inflammatory response; lymphocyte chemotaxis; protein tetramerization; phospholipase D activation; neutrophil activation; negative regulation of G-protein coupled receptor protein signaling pathway; MAPKKK cascade; positive regulation of cellular biosynthetic process; positive regulation of phosphoinositide 3-kinase cascade; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; cellular protein complex assembly; positive regulation of tyrosine phosphorylation of STAT protein; negative regulation of viral genome replication; response to cytokine stimulus; positive regulation of fever; eosinophil chemotaxis; immune response; positive regulation of neuron differentiation; positive regulation of calcium ion transport; positive regulation of defense response to virus by host; regulation of insulin secretion; positive regulation of phosphorylation; positive regulation of epithelial cell proliferation; positive regulation of cell migration; regulation of T cell activation