Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Pyrroline-5-carboxylate reductase 3 (PYCRL) Standard Curve.)
Mouse Pyrroline-5-Carboxylate Reductase 3 (PYCRL) ELISA Kit | PYCRL elisa kit
Mouse Pyrroline-5-Carboxylate Reductase 3 (PYCRL) ELISA Kit
Gene Names
Pycrl; Pycr3; 1110058B13Rik; 2700073G24Rik
Reactivity
Mouse
Synonyms
Pyrroline-5-Carboxylate Reductase 3 (PYCRL); Mouse Pyrroline-5-Carboxylate Reductase 3 (PYCRL) ELISA Kit; PYCRL; FLJ13852; PYCRL elisa kit
Reactivity
Mouse
Specificity
Mouse Pyrroline-5-carboxylate reductase 3 (PYCRL) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PYCRL. No significant cross-reactivity or interference between Mouse PYCRL and analogues was observed.
Sequence Length
274
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Pyrroline-5-carboxylate reductase 3 (PYCRL) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Pyrroline-5-carboxylate reductase 3 (PYCRL) Standard Curve.)
Related Product Information for PYCRL elisa kit
Description: This Mouse Pyrroline-5-carboxylate reductase 3 (PYCRL) ELISA Kit employs a two-site sandwich ELISA to quantitate PYCRL in samples. An antibody specific for PYCRL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PYCRL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PYCRL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYCRL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PYCRL belongs to the pyrroline-5-carboxylate reductase family. Pyrroline-5-carboxylate reductase catalyzes the the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. Pyrroline-5-carboxylate reductase(P5CR) is the enzyme that catalyzes the terminal step in the biosynthesis of proline from glutamate, the NAD(P) dependent oxidation of 1-pyrroline-5-carboxylate into proline. Pyrroline-5-carboxylate reductase, which converts pyrroline-5-carboxylate to proline, has been identified in human erythrocytes. The level of pyrroline-5-carboxylate reductase activity in these cells is comparable to the activity levels of major erythrocyte enzymes. The physiologic function of the enzyme in erythrocytes cannot be related to its function in other tissues.
Background: PYCRL belongs to the pyrroline-5-carboxylate reductase family. Pyrroline-5-carboxylate reductase catalyzes the the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. Pyrroline-5-carboxylate reductase(P5CR) is the enzyme that catalyzes the terminal step in the biosynthesis of proline from glutamate, the NAD(P) dependent oxidation of 1-pyrroline-5-carboxylate into proline. Pyrroline-5-carboxylate reductase, which converts pyrroline-5-carboxylate to proline, has been identified in human erythrocytes. The level of pyrroline-5-carboxylate reductase activity in these cells is comparable to the activity levels of major erythrocyte enzymes. The physiologic function of the enzyme in erythrocytes cannot be related to its function in other tissues.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
pyrroline-5-carboxylate reductase 3
NCBI Official Synonym Full Names
pyrroline-5-carboxylate reductase-like
NCBI Official Symbol
Pycrl
NCBI Official Synonym Symbols
Pycr3; 1110058B13Rik; 2700073G24Rik
NCBI Protein Information
pyrroline-5-carboxylate reductase 3
UniProt Protein Name
Pyrroline-5-carboxylate reductase 3
UniProt Gene Name
Pycrl
UniProt Synonym Gene Names
P5C reductase 3; P5CR 3
UniProt Entry Name
P5CR3_MOUSE
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Product Notes
The Mouse PYCRL pycrl (Catalog #AAA9717201) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717201 ELISA Kit recognizes Mouse PYCRL. It is sometimes possible for the material contained within the vial of "Pyrroline-5-Carboxylate Reductase 3 (PYCRL), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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