Mouse proteinkinaseC (PKC) ELISA Kit | PKC elisa kit

Mouse proteinkinaseC (PKC) ELISA Kit

Cat. Num. AAA264812

• Gene Names: Prkcg; PKC; PKCI; Prkc; Prkcc; RATPKCI
• Reactivity: Mouse
• Synonyms: proteinkinaseC (PKC); Mouse proteinkinaseC (PKC) ELISA Kit; PKC elisa kit

• Reactivity: Mouse
• Specificity: No cross-reaction with other factors.
• Sequence Length: 697

• Samples: Mouse serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant PKC concentration
• Assay Type: Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: 0.06 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PKC elisa kit

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Mouse PKC monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 6981400
• NCBI GeneID: 24681
• NCBI Accession #: NP_036760.1
• NCBI GenBank Nucleotide #: NM_012628.1
• Molecular Weight: 78,358 Da
• NCBI Official Full Name: protein kinase C gamma type
• NCBI Official Synonym Full Names: protein kinase C, gamma
• NCBI Official Symbol: Prkcg
• NCBI Official Synonym Symbols: PKC; PKCI; Prkc; Prkcc; RATPKCI
• NCBI Protein Information: protein kinase C gamma type; PKC-gamma; protein kinase C type I (gamma type)
• UniProt Protein Name: Protein kinase C gamma type
• UniProt Gene Name: Prkcg
• UniProt Synonym Gene Names: Pkcc; Pkcg; Prkcc; PKC-gamma
• UniProt Entry Name: KPCG_RAT

NCBI Description

enriched in the neurons of the central nervous system and the spinal cord [RGD, Feb 2006]

Uniprot Description

PKCG: a calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase. Expressed in the brain and spinal cord where its localization is restricted to neurons. Several neuronal functions, including long term potentiation and depression (LTP&LTD) specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects have been associated with neurodegenerative disorder spinocerebellar ataxia-14. Plays diverse roles in neuronal cells and eye tissues, such as regulation of the neuronal receptors GLUR4 and NMDAR1, modulation of receptors and neuronal functions related to sensitivity to opiates, pain and alcohol, mediation of synaptic function and cell survival after ischemia, and inhibition of gap junction activity after oxidative stress. Binds and phosphorylates GLUR4 glutamate receptor and regulates its function by increasing plasma membrane-associated GRIA4 expression. In primary cerebellar neurons treated with the agonist 3,5-dihyidroxyphenylglycine, functions downstream of the metabotropic glutamate receptor MGLUR5 and phosphorylates NMDAR1 receptor which plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. May be involved in the regulation of hippocampal long-term potentiation (LTP), but may be not necessary for the process of synaptic plasticity. May modulate the functionality of mu-type-opioid receptors by participating in a signaling pathway which leads to the phosphorylation and degradation of opioid receptors. May also contributes to chronic morphine-induced changes in nociceptive processing. Plays a role in neuropathic pain mechanisms and contributes to the maintenance of the allodynia pain produced by peripheral inflammation. Plays an important role in initial sensitivity and tolerance to ethanol, by mediating the behavioral effects of ethanol as well as the effects of this drug on the GABA(A) receptors. During and after cerebral ischemia modulate neurotransmission and cell survival in synaptic membranes, and is involved in insulin-induced inhibition of necrosis, an important mechanism for minimizing ischemic injury. Required for the elimination of multiple climbing fibers during innervation of Purkinje cells in developing cerebellum. Is activated in lens epithelial cells upon hydrogen peroxide treatment, and phosphorylates connexin-43, resulting in disassembly of GJA1 gap junction plaques and inhibition of gap junction activity which could provide a protective effect against oxidative stress. Phosphorylates p53 and promotes p53-dependent apoptosis in response to DNA damage. Interacts with GRIA4. Interacts with CDCP1. Interacts with TP53INP1 and p53. Expressed in Purkinje cells of the cerebellar cortex.

Protein type: Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.13; AGC group; PKC family; Alpha subfamily

Cellular Component: neuron projection; perinuclear region of cytoplasm; cytoplasm; dendrite; plasma membrane; intercellular junction; cytosol; nucleus

Molecular Function: protein binding; protein kinase C activity; zinc ion binding; protein serine/threonine/tyrosine kinase activity; calcium-dependent protein kinase C activity; ATP binding; protein kinase activity

Biological Process: positive regulation of mismatch repair; protein amino acid autophosphorylation; rhythmic process; response to morphine; response to pain; regulation of circadian rhythm; synaptic transmission; learning and/or memory; chemosensory behavior; negative regulation of protein ubiquitination; negative regulation of neuron apoptosis; negative regulation of protein catabolic process; phosphorylation; regulation of response to food