Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein naked cuticle homolog 1 (NKD1) Standard Curve.)
Mouse Protein Naked Cuticle Homolog 1 (NKD1) ELISA Kit | NKD1 elisa kit
Mouse Protein Naked Cuticle Homolog 1 (NKD1) ELISA Kit
Gene Names
Nkd1; Nkd; 2810434J10Rik; 9030215G15Rik
Reactivity
Mouse
Synonyms
Protein Naked Cuticle Homolog 1 (NKD1); Mouse Protein Naked Cuticle Homolog 1 (NKD1) ELISA Kit; NKD1; Naked1; Dvl-binding protein; naked cuticle homolog 1; naked cuticle-1; NKD1 elisa kit
Reactivity
Mouse
Specificity
Mouse Protein naked cuticle homolog 1 (NKD1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse NKD1. No significant cross-reactivity or interference between Mouse NKD1 and analogues was observed.
Sequence Length
471
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein naked cuticle homolog 1 (NKD1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein naked cuticle homolog 1 (NKD1) Standard Curve.)
Related Product Information for NKD1 elisa kit
Description: This Mouse Protein naked cuticle homolog 1 (NKD1) ELISA Kit employs a two-site sandwich ELISA to quantitate NKD1 in samples. An antibody specific for NKD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NKD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NKD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NKD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: In the mouse, Nkd is a Dishevelled-binding protein that functions as a negative regulator of the Wnt-beta-catenin-Tcf signaling pathway. The deduced 470-amino acid protein contains an EF-hand Ca(2+)-binding motif. NKD1 shares about 44% overall identity with NKD2, including more than 75% identity in 4 major homology domains, including the EF-hand motif. Northern blot analysis revealed wide expression of 5 transcripts of 9.3 to 2.3 kb in adult and fetal tissues and in cancer cell lines. Highest expression was detected in fetal kidney, followed by fetal lung, placenta, and adult liver, kidney, pancreas, and spleen. Multiple transcripts were generated through the use of alternative polyadenylation sites.
Background: In the mouse, Nkd is a Dishevelled-binding protein that functions as a negative regulator of the Wnt-beta-catenin-Tcf signaling pathway. The deduced 470-amino acid protein contains an EF-hand Ca(2+)-binding motif. NKD1 shares about 44% overall identity with NKD2, including more than 75% identity in 4 major homology domains, including the EF-hand motif. Northern blot analysis revealed wide expression of 5 transcripts of 9.3 to 2.3 kb in adult and fetal tissues and in cancer cell lines. Highest expression was detected in fetal kidney, followed by fetal lung, placenta, and adult liver, kidney, pancreas, and spleen. Multiple transcripts were generated through the use of alternative polyadenylation sites.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein naked cuticle homolog 1 isoform 1
NCBI Official Synonym Full Names
naked cuticle 1
NCBI Official Symbol
Nkd1
NCBI Official Synonym Symbols
Nkd; 2810434J10Rik; 9030215G15Rik
NCBI Protein Information
protein naked cuticle homolog 1
UniProt Protein Name
Protein naked cuticle homolog 1
Protein Family
UniProt Gene Name
Nkd1
UniProt Synonym Gene Names
Naked-1; mNkd; mNkd1
UniProt Entry Name
NKD1_MOUSE
Research Articles on NKD1
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Product Notes
The Mouse NKD1 nkd1 (Catalog #AAA9718069) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718069 ELISA Kit recognizes Mouse NKD1. It is sometimes possible for the material contained within the vial of "Protein Naked Cuticle Homolog 1 (NKD1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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