Mouse Protein Kinase, cGMP Dependent Type II (PRKG2) ELISA Kit | PRKG2 elisa kit

Mouse Protein Kinase, cGMP Dependent Type II (PRKG2) ELISA Kit

Cat. Num. AAA2706367

• Gene Names: PRKG2; cGK2; cGKII; PRKGR2
• Reactivity: Mouse
• Synonyms: Protein Kinase; cGMP Dependent Type II (PRKG2); Mouse Protein Kinase; cGMP Dependent Type II (PRKG2) ELISA Kit; PRKGR2; cGKII; cGMP-dependent protein kinase 2; PRKG2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Protein Kinase, cGMP Dependent Type II (PRKG2).; No significant cross-reactivity or interference between Protein Kinase, cGMP Dependent Type II (PRKG2) and analogues was observed.
• Sequence Length: 762

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 3.12-200ng/mL
• Sensitivity: < 1.37ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase, cGMP Dependent Type II (PRKG2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase, cGMP Dependent Type II (PRKG2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PRKG2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase, cGMP Dependent Type II (PRKG2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase, cGMP Dependent Type II (PRKG2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase, cGMP Dependent Type II (PRKG2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase, cGMP Dependent Type II (PRKG2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119626272
• NCBI GeneID: 5593
• Molecular Weight: 87,432 Da
• NCBI Official Full Name: protein kinase, cGMP-dependent, type II
• NCBI Official Synonym Full Names: protein kinase, cGMP-dependent, type II
• NCBI Official Symbol: PRKG2
• NCBI Official Synonym Symbols: cGK2; cGKII; PRKGR2
• NCBI Protein Information: cGMP-dependent protein kinase 2
• UniProt Protein Name: cGMP-dependent protein kinase
• Protein Family: cGMP-dependent protein kinase
• UniProt Gene Name: PRKG2

NCBI Description

This gene encodes a protein that belongs to the serine/threonine protein kinase family of proteins. The encoded protein plays a role in the regulation of fluid balance in the intestine. A similar protein in mouse is thought to regulate differentiation and proliferation of cells in the colon. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]