Mouse Protein Kinase B Alpha (PKBa) ELISA Kit | PKBa elisa kit

Mouse Protein Kinase B Alpha (PKBa) ELISA Kit

Cat. Num. AAA2000129

• Gene Names: Akt1; Akt; PKB; Rac; PKB/Akt; PKBalpha
• Reactivity: Mouse
• Synonyms: Protein Kinase B Alpha (PKBa); Mouse Protein Kinase B Alpha (PKBa) ELISA Kit; AKT1; AKT; PKB-A; Proto-oncogene c-Akt; RAC-Alpha Serine/Threonine-Protein Kinase; V-akt Murine Thymoma Viral Oncogene Homolog 1; PKBa elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Protein Kinase B Alpha (PKBa).; No significant cross-reactivity or interference between Protein Kinase B Alpha (PKBa) and analogues was observed.
• Sequence Length: 480

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase B Alpha (PKBa) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase B Alpha (PKBa) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PKBa elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase B Alpha (PKBa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase B Alpha (PKBa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase B Alpha (PKBa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase B Alpha (PKBa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PKBa elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 22653384
• NCBI GeneID: 11651
• UniProt Accession #: P31750; Q62274; Q6GSA6
• Molecular Weight: 55,707 Da
• NCBI Official Full Name: protein kinase B-alpha
• NCBI Official Synonym Full Names: thymoma viral proto-oncogene 1
• NCBI Official Symbol: Akt1
• NCBI Official Synonym Symbols: Akt; PKB; Rac; PKB/Akt; PKBalpha
• NCBI Protein Information: RAC-alpha serine/threonine-protein kinase
• UniProt Protein Name: RAC-alpha serine/threonine-protein kinase
• Protein Family: RAC family serine/threonine-protein kinase
• UniProt Gene Name: Akt1
• UniProt Synonym Gene Names: Akt; Rac; PKB; PKB alpha
• UniProt Entry Name: AKT1_MOUSE

NCBI Description

This gene encodes the founding member of the Akt serine-threonine protein kinase gene family that also includes Akt2 and Akt3. This kinase is a major downstream effector of the phosphatidylinositol 3-kinase (PI3K) pathway that mediates the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). It is activated through recruitment to cellular membranes by PI3K lipid products and by phosphorylation by 3-phosphoinositide dependent kinase-1. It then further phosphorylates different downstream proteins in response to various extracellular signals and thus plays a pivotal role in mediating a variety of cellular processes, such as glucose metabolism, glycogen biosynthesis, protein synthesis and turn over, inflammatory response, cell survival (anti-apoptosis) and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

Akt1: an oncogenic AGC kinase that plays a critical role in regulating cell survival and metabolism in many different signaling pathways. Dual phosphorylation is required for its activation. T308 is phosphorylated by PDK1 in the PI3 kinase pathway, and S473 is phosphorylated by mTOR in the mTORC2 pathway. The 'Lys-63'-linked ubiquitination of AKT1 by TRAF6 is important for its translocation to the plasma membrane, phosphorylation, and activation. When Akt is fully phosphorylated it translocates into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its proteosomal degradation. Hyperactive or overexpressed in a number of cancers including breast, prostate, lung, pancreatic, liver, ovarian and colorectal. Over 160 protein substrates are known including many that regulate transcription, metabolism, apoptosis, cell cycle, and growth.

Protein type: Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Oncoprotein; AGC group; AKT family

Cellular Component: microtubule cytoskeleton; nucleoplasm; protein complex; membrane; mitochondrion; cytoplasm; plasma membrane; spindle; intercellular junction; intracellular; cytosol; nucleus

Molecular Function: identical protein binding; protein phosphatase 2A binding; nitric-oxide synthase regulator activity; nucleotide binding; protein kinase binding; protein kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; enzyme binding; protein kinase C binding; transferase activity, transferring phosphorus-containing groups; protein serine/threonine/tyrosine kinase activity; kinase activity; phosphatidylinositol-3,4-bisphosphate binding; ATP binding

Biological Process: glycogen metabolic process; negative regulation of JNK cascade; regulation of myelination; positive regulation of nitric oxide biosynthetic process; positive regulation of apoptosis; multicellular organismal development; protein ubiquitination; glucose homeostasis; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; protein amino acid phosphorylation; regulation of cell migration; germ cell development; positive regulation of glucose import; regulation of protein localization; cell projection organization and biogenesis; protein catabolic process; maternal placenta development; response to food; nervous system development; glycogen biosynthetic process; positive regulation of nitric-oxide synthase activity; positive regulation of blood vessel endothelial cell migration; carbohydrate transport; glucose metabolic process; positive regulation of lipid biosynthetic process; positive regulation of cell growth; cellular response to insulin stimulus; insulin-like growth factor receptor signaling pathway; carbohydrate metabolic process; positive regulation of fat cell differentiation; striated muscle cell differentiation; negative regulation of protein kinase activity; positive regulation of transcription factor activity; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; anagen; negative regulation of apoptosis; negative regulation of autophagy; negative regulation of fatty acid beta-oxidation; translation; apoptosis; positive regulation of glycogen biosynthetic process; regulation of glycogen biosynthetic process; positive regulation of cellular protein metabolic process; response to hormone stimulus; negative regulation of cell size; glucose transport; negative regulation of caspase activity; signal transduction; regulation of translation; apoptotic mitochondrial changes; transport; lipopolysaccharide-mediated signaling pathway; protein kinase B signaling cascade; inflammatory response; activated T cell apoptosis; aging; negative regulation of proteolysis; myelin maintenance in the peripheral nervous system; cytoskeleton organization and biogenesis; positive regulation of peptidyl-serine phosphorylation; osteoblast differentiation; peptidyl-serine phosphorylation; protein import into nucleus, translocation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of vasoconstriction; insulin receptor signaling pathway; positive regulation of protein amino acid phosphorylation; phosphorylation