Human Small Dense Low Density Lipoprotein ELISA Kit | sdLDL elisa kit

Human Small Dense Low Density Lipoprotein ELISA Kit

• Reactivity: Human
• Synonyms: Small Dense Low Density Lipoprotein; Human Small Dense Low Density Lipoprotein ELISA Kit; sdLDL; sdLDL elisa kit

• Reactivity: Human
• Specificity: Specifically recognize sdLDL, no obvious cross reaction with other analogues

• Assay Type: Sandwich
• Samples: Serum, plasma, cell culture supernatant and other biological samples
• Intra-assay Precision: Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
• Inter-assay Precision: Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for sdLDL elisa kit

Background: Small dense low-density lipoprotein (sdLDL) is the smaller and denser fraction of low-density lipoprotein (LDL). Recent studies have found that sdLDL has a stronger atherogenic ability than ordinary LDL, and it has been included as one of the new important risk factors for cardiovascular disease.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti sdLDL antibody was precoated onto the 96-well plate. The biotin conjugated anti sdLDL antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with sdLDL conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of sdLDL in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.