Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein disulfide-isomerase A4 (PDIA4) Standard Curve.)
Mouse Protein Disulfide-Isomerase A4 (PDIA4) ELISA Kit | PDIA4 elisa kit
Mouse Protein Disulfide-Isomerase A4 (PDIA4) ELISA Kit
Gene Names
Pdia4; Cai; Erp72; ERp-72; AI987846
Reactivity
Mouse
Synonyms
Protein Disulfide-Isomerase A4 (PDIA4); Mouse Protein Disulfide-Isomerase A4 (PDIA4) ELISA Kit; PDIA4; ERP70; ERP72; protein disulfide isomerase A4; protein disulfide isomerase related protein (calcium-binding protein; intestinal-related); protein disulfide isomerase-associated 4; PDIA4 elisa kit
Reactivity
Mouse
Specificity
Mouse Protein disulfide-isomerase A4 (PDIA4) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PDIA4. No significant cross-reactivity or interference between Mouse PDIA4 and analogues was observed.
Sequence Length
641
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein disulfide-isomerase A4 (PDIA4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein disulfide-isomerase A4 (PDIA4) Standard Curve.)
Related Product Information for PDIA4 elisa kit
Description: This Mouse Protein disulfide-isomerase A4 (PDIA4) ELISA Kit employs a two-site sandwich ELISA to quantitate PDIA4 in samples. An antibody specific for PDIA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDIA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDIA4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDIA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Protein Disulfide Isomerase A4is an enzyme in the endoplasmic reticulum in eukaryotes or periplasmic space of prokaryotes that catalyzes the formation and breakage of disulfide bonds between cysteine residues within proteins as they fold. This allows proteins to quickly find the correct arrangement of disulfide bonds in their fully-folded state, and therefore the enzyme acts to catalyze protein folding. The reduced (dithiol) form of PDI is able to catalyse a reduction of mispaired thiol residues of a particular substrate, acting as an isomerase. Therefore, PDI is capable of catalyzing the posttranslational modification disulfide exchange. Such exchange reactions can occur intramolecularly, leading to the rearrangement of disulfide bonds in a single protein.
Background: Protein Disulfide Isomerase A4is an enzyme in the endoplasmic reticulum in eukaryotes or periplasmic space of prokaryotes that catalyzes the formation and breakage of disulfide bonds between cysteine residues within proteins as they fold. This allows proteins to quickly find the correct arrangement of disulfide bonds in their fully-folded state, and therefore the enzyme acts to catalyze protein folding. The reduced (dithiol) form of PDI is able to catalyse a reduction of mispaired thiol residues of a particular substrate, acting as an isomerase. Therefore, PDI is capable of catalyzing the posttranslational modification disulfide exchange. Such exchange reactions can occur intramolecularly, leading to the rearrangement of disulfide bonds in a single protein.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein disulfide-isomerase A4 isoform 2
NCBI Official Synonym Full Names
protein disulfide isomerase associated 4
NCBI Official Symbol
Pdia4
NCBI Official Synonym Symbols
Cai; Erp72; ERp-72; AI987846
NCBI Protein Information
protein disulfide-isomerase A4
UniProt Protein Name
Protein disulfide-isomerase A4
Protein Family
UniProt Gene Name
Pdia4
UniProt Synonym Gene Names
Cai; Erp72; ER protein 72; ERp-72; ERp72
UniProt Entry Name
PDIA4_MOUSE
Uniprot Description
PDIA4: Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Belongs to the protein disulfide isomerase family.
Protein type: Chaperone; EC 5.3.4.1; Endoplasmic reticulum; Isomerase
Cellular Component: cell surface; endoplasmic reticulum; endoplasmic reticulum lumen; smooth endoplasmic reticulum
Molecular Function: isomerase activity; protein disulfide isomerase activity
Biological Process: cell redox homeostasis; protein folding
Research Articles on PDIA4
Similar Products
Product Notes
The Mouse PDIA4 pdia4 (Catalog #AAA9717385) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717385 ELISA Kit recognizes Mouse PDIA4. It is sometimes possible for the material contained within the vial of "Protein Disulfide-Isomerase A4 (PDIA4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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