Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein AF-10 (MLLT10) Standard Curve.)
Mouse Protein AF-10 (MLLT10) ELISA Kit | MLLT10 elisa kit
Mouse Protein AF-10 (MLLT10) ELISA Kit
Gene Names
Mllt10; Af10; mKIAA4140; B130021D15Rik; D630001B22Rik
Reactivity
Mouse
Synonyms
Protein AF-10 (MLLT10); Mouse Protein AF-10 (MLLT10) ELISA Kit; MLLT10; RP11-399C16.2; AF10; DKFZp686E10210; MGC75086; ALL-1 fused gene from chromosome 10; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocated to; 10; myeloid/lymphoid or mixed-linea; MLLT10 elisa kit
Reactivity
Mouse
Specificity
Mouse Protein AF-10 (MLLT10) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MLLT10. No significant cross-reactivity or interference between Mouse MLLT10 and analogues was observed.
Sequence Length
1068
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein AF-10 (MLLT10) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Protein AF-10 (MLLT10) Standard Curve.)
Related Product Information for MLLT10 elisa kit
Description: This Mouse Protein AF-10 (MLLT10) ELISA Kit employs a two-site sandwich ELISA to quantitate MLLT10 in samples. An antibody specific for MLLT10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MLLT10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MLLT10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MLLT10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The 11q23 gene involved in the translocations is MLL. In both patients, the translocation fused MLL to a gene that the authors designated AF10. The AF10 gene encodes a predicted 1,027-amino acid protein containing an N-terminal zinc finger and a C-terminal leucine zipper domain that are highly homologous to similar domains in AF17 and BR140. Northern blot analysis showed that AF10 is expressed as a 5.5-kb mRNA in many tissues with highest expression in testis, and has an alternatively spliced exon.all known translocations involving MLL and AF10 or AF17 have involved breakpoints between the zinc finger and leucine zipper domains, and have yielded fusion proteins in which the MLL zinc fingers were replaced with leucine zipper regions.
Background: The 11q23 gene involved in the translocations is MLL. In both patients, the translocation fused MLL to a gene that the authors designated AF10. The AF10 gene encodes a predicted 1,027-amino acid protein containing an N-terminal zinc finger and a C-terminal leucine zipper domain that are highly homologous to similar domains in AF17 and BR140. Northern blot analysis showed that AF10 is expressed as a 5.5-kb mRNA in many tissues with highest expression in testis, and has an alternatively spliced exon.all known translocations involving MLL and AF10 or AF17 have involved breakpoints between the zinc finger and leucine zipper domains, and have yielded fusion proteins in which the MLL zinc fingers were replaced with leucine zipper regions.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein AF-10 isoform 1
NCBI Official Synonym Full Names
myeloid/lymphoid or mixed-lineage leukemia; translocated to, 10
NCBI Official Symbol
Mllt10
NCBI Official Synonym Symbols
Af10; mKIAA4140; B130021D15Rik; D630001B22Rik
NCBI Protein Information
protein AF-10
UniProt Protein Name
Protein AF-10
Protein Family
UniProt Gene Name
Mllt10
UniProt Synonym Gene Names
Af10
Uniprot Description
MLLT10: Probably involved in transcriptional regulation. In vitro or as fusion protein with MLL has transactivation activity. Binds to cruciform DNA. A chromosomal aberration involving MLLT10 is associated with acute leukemias. Translocation t(10;11)(p12;q23) with MLL/HRX. The result is a rogue activator protein. A chromosomal aberration involving MLLT10 is associated with diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with PICALM. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Oncoprotein; Transcription factor
Chromosomal Location of Human Ortholog: 2|2 A3
Cellular Component: cytosol; nucleoplasm; nucleus; protein complex
Molecular Function: metal ion binding
Biological Process: positive regulation of transcription from RNA polymerase II promoter
Research Articles on MLLT10
Similar Products
Product Notes
The Mouse MLLT10 mllt10 (Catalog #AAA9718075) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718075 ELISA Kit recognizes Mouse MLLT10. It is sometimes possible for the material contained within the vial of "Protein AF-10 (MLLT10), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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