Western Blot (WB)
(Western blot analysis of MLLT10 in K562 cell line lysates (35ug/lane). MLLT10 (arrow) was detected using the purified Pab.(2ug/ml))
Rabbit anti-Human MLLT10 (AF10) Polyclonal Antibody | anti-MLLT10 antibody
MLLT10 (AF10) Antibody (Center)
Western Blot (WB)
(Western blot analysis of MLLT10 in K562 cell line lysates (35ug/lane). MLLT10 (arrow) was detected using the purified Pab.(2ug/ml))
Western Blot (WB)
(Western blot analysis of MLLT10 in K562 cell line lysates (35ug/lane). MLLT10 (arrow) was detected using the purified Pab.(2ug/ml))
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with MLLT10 (AF10) Antibody (Center). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MLLT10 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). MLLT10 immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with MLLT10 (AF10) Antibody (Center). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MLLT10 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). MLLT10 immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.)
Roll, P., et al., Cancer Genet. Cytogenet. 135(2):187-191 (2002).
Nakamura, T., et al., Mol. Cell 10(5):1119-1128 (2002).
Debernardi, S., et al., Blood 99(1):275-281 (2002).
Cai, Y., et al., Mol. Reprod. Dev. 61(1):126-134 (2002).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a transcription factor and has been identified as a partner gene involved in several chromosomal rearrangements resulting in various leukemias. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]
Uniprot Description
MLLT10: Probably involved in transcriptional regulation. In vitro or as fusion protein with MLL has transactivation activity. Binds to cruciform DNA. A chromosomal aberration involving MLLT10 is associated with acute leukemias. Translocation t(10;11)(p12;q23) with MLL/HRX. The result is a rogue activator protein. A chromosomal aberration involving MLLT10 is associated with diffuse histiocytic lymphomas. Translocation t(10;11)(p13;q14) with PICALM. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Oncoprotein; Transcription factor
Chromosomal Location of Human Ortholog: 10p12
Cellular Component: nucleoplasm; cytoplasm
Molecular Function: protein binding; DNA binding; zinc ion binding; transcription factor activity
Biological Process: transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter
Disease: Leukemia, Acute Myeloid
Research Articles on MLLT10
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Product Notes
The MLLT10 mllt10 (Catalog #AAA9201433) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 294-323. The MLLT10 (AF10) Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MLLT10 (AF10) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunofluorescence (IF). WB~~1:1000. Researchers should empirically determine the suitability of the MLLT10 mllt10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MLLT10 (AF10), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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