Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Probable prolyl-tRNA synthetase, mitochondrial (PARS2) Standard Curve.)
Mouse Probable prolyl-tRNA Synthetase, Mitochondrial (PARS2) ELISA Kit | PARS2 elisa kit
Mouse Probable prolyl-tRNA Synthetase, Mitochondrial (PARS2) ELISA Kit
Gene Names
Pars2; BC027073
Reactivity
Mouse
Synonyms
Probable prolyl-tRNA Synthetase; Mitochondrial (PARS2); Mouse Probable prolyl-tRNA Synthetase; Mitochondrial (PARS2) ELISA Kit; PARS2; DKFZp727A071; MGC14416; MGC19467; MT-PRORS; proline tRNA ligase 2; mitochondrial (putative); prolyl-tRNA synthetase (mitochondrial)(putative); prolyl-tRNA synthetase; PARS2 elisa kit
Reactivity
Mouse
Specificity
Mouse Probable prolyl-tRNA synthetase, mitochondrial (PARS2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PARS2. No significant cross-reactivity or interference between Mouse PARS2 and analogues was observed.
Sequence Length
475
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Probable prolyl-tRNA synthetase, mitochondrial (PARS2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Probable prolyl-tRNA synthetase, mitochondrial (PARS2) Standard Curve.)
Related Product Information for PARS2 elisa kit
Description: This Mouse Probable prolyl-tRNA synthetase, mitochondrial (PARS2) ELISA Kit employs a two-site sandwich ELISA to quantitate PARS2 in samples. An antibody specific for PARS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PARS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PARS2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PARS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: By searching a database for tRNA synthetases, Bonnefond et al. (2005) identified PARS2, which they called MT-PRORS. The deduced 475-amino acid protein has a 47-amino acid mitochondrial targeting signal, resulting in a mature protein of 428 amino acids. PARS2 is a class II amino acid tRNA synthetase, with a C-terminal active-site domain linked to an N-terminal anticodon-binding domain by a short hinge. PARS2 shares no significant similarity with its cytosolic counterpart, EPRS. Bonnefond et al. (2005) determined that the PARS2 gene contains 1 exon and spans 1.4 kb. By genomic sequence analysis, Bonnefond et al. (2005) mapped the PARS2 gene to chromosome 1.
Background: By searching a database for tRNA synthetases, Bonnefond et al. (2005) identified PARS2, which they called MT-PRORS. The deduced 475-amino acid protein has a 47-amino acid mitochondrial targeting signal, resulting in a mature protein of 428 amino acids. PARS2 is a class II amino acid tRNA synthetase, with a C-terminal active-site domain linked to an N-terminal anticodon-binding domain by a short hinge. PARS2 shares no significant similarity with its cytosolic counterpart, EPRS. Bonnefond et al. (2005) determined that the PARS2 gene contains 1 exon and spans 1.4 kb. By genomic sequence analysis, Bonnefond et al. (2005) mapped the PARS2 gene to chromosome 1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
probable proline--tRNA ligase, mitochondrial isoform 1
NCBI Official Synonym Full Names
prolyl-tRNA synthetase (mitochondrial)(putative)
NCBI Official Symbol
Pars2
NCBI Official Synonym Symbols
BC027073
NCBI Protein Information
probable proline--tRNA ligase, mitochondrial
UniProt Protein Name
Probable proline--tRNA ligase, mitochondrial
UniProt Gene Name
Pars2
UniProt Synonym Gene Names
ProRS
UniProt Entry Name
SYPM_MOUSE
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Product Notes
The Mouse PARS2 pars2 (Catalog #AAA9718064) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9718064 ELISA Kit recognizes Mouse PARS2. It is sometimes possible for the material contained within the vial of "Probable prolyl-tRNA Synthetase, Mitochondrial (PARS2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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