Mouse PRKAa1 ELISA Kit | PRKAa1 elisa kit

Mouse PRKAa1 (Protein Kinase, AMP Activated Alpha 1) ELISA Kit

Cat. Num. AAA2500651

• Gene Names: PRKAA1; AMPK; AMPKa1
• Reactivity: Mouse
• Synonyms: PRKAa1; Mouse PRKAa1 (Protein Kinase; AMP Activated Alpha 1) ELISA Kit; PRKAa1 elisa kit

• Reactivity: Mouse
• Specificity: This kit recognizes natural and recombinant Mouse PRKAa1. No significant cross-reactivity or interference between Mouse PRKAa1 and analogues was observed.
• Sequence Length: 550

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 1.563-100ng/mL
• Sensitivity: Min: 0.938ng/mL; Max: 100ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PRKAa1 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse PRKAa1 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PRKAa1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PRKAa1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PRKAa1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PRKAa1. You can calculate the concentration of PRKAa1 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 27469582
• NCBI GeneID: 5562
• UniProt Accession #: Q13131; O00286; Q5D0E1; Q86VS1; Q9UNQ4; A8MTQ6; B2R7E1
• Molecular Weight: 65,523 Da
• NCBI Official Full Name: PRKAA1 protein
• NCBI Official Synonym Full Names: protein kinase, AMP-activated, alpha 1 catalytic subunit
• NCBI Official Symbol: PRKAA1
• NCBI Official Synonym Symbols: AMPK; AMPKa1
• NCBI Protein Information: 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; ACACA kinase; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK alpha 1; AMPK subunit alpha-1; HMGCR kinase; acetyl-CoA carboxylase kinase; hydroxymethylglutaryl-CoA reductase kinase; tau-protein kinase PRKAA1
• UniProt Protein Name: 5'-AMP-activated protein kinase catalytic subunit alpha-1
• UniProt Gene Name: PRKAA1
• UniProt Synonym Gene Names: AMPK1; AMPK subunit alpha-1; ACACA kinase; HMGCR kinase
• UniProt Entry Name: AAPK1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

AMPKA1: a catalytic subunit of AMP-activated protein kinase (AMPK). Acts as an energy sensor, playing a key role in regulating cellular energy metabolism. A protein kinase of the CAMKL family whose activation is regulated by the balance between ADP/AMP/ATP, and intracellular Ca(2+) levels. Acts as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-ADP and -AMP rise in response to fuel limitation and/or hypoxia. Activates energy-producing pathways and inhibits energy-consuming processes. Restores ATP levels in cells by switching off anabolic and switching on catabolic pathways. Activated primarily by rising ADP levels and not, as previously thought, solely by AMP. AMPK resembles an adenylate charge regulatory system in which anabolic and catabolic pathways are regulated by adenine nucleotide ratios. Acts via direct phosphorylation of a number of metabolic enzymes and transcription regulators. Activated by at least two distinct upstream kinases: the tumor suppressor LKB1 and CaMKK2. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. AMPK is a heterotrimer of an alpha catalytic subunit (AMPKA1 or -2), a beta (AMPKB1 or -2) and a gamma non-catalytic subunit (AMPKG1, -2 or -3). Different possible combinations of subunits give rise to 12 different holoenzymes. Binding of ADP or AMP to non-catalytic gamma subunit (PRKAG1, -2 or -3) results in allosteric activation. AMPK is activated by antihyperglycemic drug metformin, a drug prescribed to patients with type 2 diabetes: in vivo, metformin seems to mainly inhibit liver gluconeogenesis. However, metformin can be used to activate AMPK in muscle and other cells in culture or ex vivo. Selectively inhibited by compound C (6-[4-(2-Piperidin-1-yl-ethoxy)-phenyl)]-3-pyridin-4-yl-pyyrazolo[1,5-a] pyrimidine. Activated by resveratrol, a natural polyphenol present in red wine, and S17834, a synthetic polyphenol. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, CAMK; Kinase, protein; EC 2.7.11.27; EC 2.7.11.1; Autophagy; EC 2.7.11.31; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.26; CAMK group; CAMKL family; AMPK subfamily

Chromosomal Location of Human Ortholog: 5p12

Cellular Component: nucleoplasm; apical plasma membrane; cytoplasm; intracellular; nucleus; cytosol; AMP-activated protein kinase complex

Molecular Function: protein C-terminus binding; AMP-activated protein kinase activity; cAMP-dependent protein kinase activity; metal ion binding; [acetyl-CoA carboxylase] kinase activity; histone serine kinase activity; protein kinase activity; protein binding; tau-protein kinase activity; chromatin binding; kinase binding; ATP binding; [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity

Biological Process: protein heterooligomerization; activation of MAPK activity; lipid biosynthetic process; rhythmic process; positive regulation of cholesterol biosynthetic process; glucose homeostasis; signal transduction; protein amino acid phosphorylation; response to caffeine; cellular response to glucose starvation; cellular response to nutrient levels; regulation of transcription, DNA-dependent; negative regulation of lipid catabolic process; positive regulation of cell proliferation; response to gamma radiation; cell cycle arrest; negative regulation of glucosylceramide biosynthetic process; fatty acid biosynthetic process; positive regulation of autophagy; response to UV; negative regulation of TOR signaling pathway; Wnt receptor signaling pathway; transcription, DNA-dependent; glucose metabolic process; regulation of circadian rhythm; cholesterol biosynthetic process; fatty acid homeostasis; cold acclimation; positive regulation of glycolysis; response to hypoxia; insulin receptor signaling pathway; autophagy; fatty acid oxidation; response to activity; negative regulation of apoptosis